Healthy Adults
Conditions
Keywords
Bioavailability, Polyphenols, Dietary fibre, Phenolic acids
Brief summary
This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. The investigators hypothesize that combination of polyphenolics from a soup rich in rutin and quercitin and the non-digestible carbohydrate (NDC) inulin will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomized order: Tomato, onion and lovage soup (high polyphenol food), Inulin (NDC) or Mixture of tomato, onion and lovage soup and inulin. During each feeding study, urine, blood and stool samples will be collected at regular intervals for the duration of 24 hrs after consumption of test food. Participants will be asked to follow a low polyphenol diet for 2 days prior to the feeding study.
Detailed description
Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols. Colonic bacteria are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as inulin to short chain fatty acids. It is likely that there are key interactions in the colonic bacterial metabolism of NDC and phenolics. The investigators hypothesize that combination of polyphenolics (in onions, tomatoes and lovage) with inulin (NDC) will increase the urinary output of bioactive phenolic acids.
Interventions
DIETARY_SUPPLEMENTTomato, onion & lovage soup with inulin
Source of polyphenols and non digestible carbohydrate
DIETARY_SUPPLEMENTTomato, onion & lovage soup
Source of polyphenols
DIETARY_SUPPLEMENTInulin
Source of non digestible carbohydrate
Sponsors
Scottish Universities Environmental Research Centre
University of Glasgow
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Acute human bioavailability studies with a cross-over design. Subjects will be given a soup or drink in one of three combinations 1. Tomato onion and lovage soup 2. Inulin 3. Mixture of tomato onion and lovage soup with inulin
Eligibility
Sex/Gender
ALL
Age
20 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
• Self-reported healthy adults
Exclusion criteria
* Antibiotic use within the last 3 months * Identified gastro-intestinal diseases * On prescribed medication other than the contraceptive pill * Pregnant or breastfeeding. * Diagnosed as anaemic * Allergic to paracetamol or any food
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Phenolic acids bioavailability | 0-24 hrs | Urine excretion of phenolic acids (µg) will be measured with GC-MS |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Glycaemic measurements | 0-8 hrs | Plasma glucose (mmol/L), insulin (mU/L) will be measured by commercial kits |
| Appetite hormones measurements | 0-8 hrs | Appetite hormone (PYY) levels in plasma (pg/mL) will be measured by commercial Elisa kit |
| Mouth to caecum transit time | 0-8 hrs | Mouth to caecum transit time (in hours/mins) will be calculated from sustained rise in breath hydrogen level measurements by hydrogen monitor |
| Gastric emptying time | 0-6 hrs | Gastric emptying time (in hours/mins) will be estimated using kinetics of plasma paracetamol levels measured by acetaminophen assay kits |
Countries
United Kingdom
Outcome results
None listed