Type2 Diabetes
Conditions
Keywords
chylomicrons, Very Low Density Lipoprotein, saturated fat, platelet function, type 2 diabetes, post prandial phase
Brief summary
To investigate in vitro the effect of triglyceride rich lipoproteins (TGRL) obtained in type 2 diabetic women in the fasting state and following a randomized isocaloric lipid rich breakfast on control human platelets Randomized Comparison of butter versus chocolate spread in 2 groups of 15 patients
Interventions
OTHERbutter based breakfast
ingestion of proteins : 5 g, lipids 20 g, glucides 57 g
OTHERChocolate spread based breakfast
ingestion of proteins 7 g, lipids 20 g, glucides 56 g
Sponsors
Hospices Civils de Lyon
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
50 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* Menopausal women * HbA1c ≥6,5 ou ≤10% * 27 ≤ BMI ≤40 kg/m2
Exclusion criteria
* Insulin therapy * fibrates or Omega 3 fatty acids supplements * Hormonal replacement therapy of menopause * Glycemia \> 16 mmol/l * Alcohol abuse (≥3 Unit/day) * Special dietary habits * History of bariatric surgery
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| fasting thromboxane B2 concentration | Hour 0 | ex vivo experiment : TGRL obtained by preparative Unesterified cholesterol (UC) incubated with control human platelets |
| after breakfast thromboxane B2 concentration | 4 hours | ex vivo experiment : TGRL obtained by preparative Unesterified cholesterol (UC) incubated with control human platelets |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| measurement of fasting platelet activation by turbidimetric method | Hour 0 | — |
| measurement of post prandial platelet activation by turbidimetric method | 4 Hours | — |
| fasting lipidomic composition of triglyceride rich lipoproteins | Hour 0 | Characterization and comparison of LRT lipidomics in fasting DT2 patients and following the ingestion of a high-fat breakfast according to their source (short and medium chain fatty acids (butter) versus palmitic acid (palm oil present in spreads). |
| post prandial lipidomic composition of triglyceride rich lipoproteins | 4 Hours | Characterization and comparison of LRT lipidomics in fasting DT2 patients and following the ingestion of a high-fat breakfast according to their source (short and medium chain fatty acids (butter) versus palmitic acid (palm oil present in spreads). |
Countries
France
Outcome results
None listed