Colorectal Neoplasms
Conditions
Keywords
Microsatellite Instability, Circulating Tumor DNA, Colorectal Neoplasms
Brief summary
The method to analyze the microsatellite instability (MSI) status by next-generation sequencing (NGS) has been established to assess the deficiency of DNA mismatch repair (MMR) system. The aim of our study is to evaluate the feasibility and reliability of this NGS method by testing the circulating tumor DNA (ctDNA) in blood sample of advanced colorectal cancer patients. If the result is positive, the MSI status could be easily learned without the acquisition of tissue samples.
Interventions
None listed
Sponsors
Shanghai Minimally Invasive Surgery Center
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* a biopsy proven histological diagnosis of colorectal carcinoma * MSI status of tissue sample known by IHC or PCR method * willing to participate and informed consent signed
Exclusion criteria
* history of other malignant tumors * patients with inflammatory bowel disease * patients with familial adenomatous polyposis * patients treated by surgery or adjuvant treatment * pregnant or lactating women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| MSI status in blood sample | through study completion, an average of 1 year | ctDNA and leucocyte are extracted from blood sample for MSI detection by ColonCore NGS panel (Burning Rock, Guangzhou, China). There are three types of MSI status, MSI-H, MSI-L and MSS. |
Countries
China
Contacts
Primary ContactZhenghao CAI
Outcome results
None listed