Scleroderma, Systemic
Conditions
Keywords
systemic sclerosis, B lymphocyte
Brief summary
B Lymphocytes are thought to play an important role in the pathophysiology of systemic sclerosis. In this study, the profibrosing role of B lymphocytes of patients with systemic sclerosis will be evaluated.
Detailed description
B Lymphocytes of patients with systemic sclerosis will be assessed in term of interaction with fibroblasts from patients with systemic sclerosis and compared to healthy controls.
Interventions
PROCEDUREskin biopsy
A skin biopsy will be performed in 10 patients in the form of 2 punches of 5 mm in the injured area in aseptic condition with a Rodnan score. In this study, 10 skin biopsies are needed, and will be performed on the first 10 patients accepting this biopsy.
OTHERBlood punction
1 blood punction during the normal pathway of care of the patient. This punction will be used to perform the extraction of B lymphocyte
Sponsors
University Hospital, Lille
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
patients * systemic sclerosis according to ACR-EULAR 2013 * written consent * available insurance Healthy blood and healthy biopsy controls * written consent * available insurance
Exclusion criteria
patients * overlap with other connective tissue diseases * immunosuppressants in the past 12 months * corticosteroids ≥10mg/d * pregnancy or breast feeding Healthy blood and healthy biopsy controls * systemic sclerosis according to ACR-EULAR 2013 * another connective tissue diseases * immunosuppressants in the past 12 months * corticosteroids ≥10mg/d * pregnancy or breast feeding * With dysimmune inflammatory diseases (except thyroiditis) * Presenting an infection in progress or within the previous 15 days
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fibroblast transcriptomic : myofibroblast signature | 36 months | expression of alpha Smooth Muscle Actin |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Fibroblast transcriptomic | 36 months | expression of COL1A1, COL1A2, COL3A1 by quantitative RT-PCR |
| Difference in collagen infiltration by PicroSirius red staining between the skin biopsies of the two groups | 36 months | — |
| Difference in the inflammatory infiltrate by immunohistological markings between the skin biopsies of the two groups | 36 months | — |
Countries
France
Outcome results
None listed