Lung Cancer, Surgery
Conditions
Keywords
Lymphopenia, Lung cancer surgery, TIM-3, PD-1, CTLA-4, lymphocytes, CD4, CD8
Brief summary
Postoperative pneumonia is one of the most common complications after lung cancer surgery and associated with a morbidity and mortality. Postoperative lymphopenia has been recently identified as one of risk factors for postoperative pneumonia. According to recent studies in polytrauma, cancer or septic shock, T cells dysfunction may be related to high expression of inhibitory receptors on lymphocytes.
Detailed description
This study will investigate prospectively TIM-3, PD-1 and CTLA4 expression on lymphocytes T cells before and after lung cancer surgery. Patients ≥ 18 years with no history of immunosuppressive state will be included.
Interventions
BIOLOGICALBlood samples
Blood samples will be collected in patients undergoing lung cancer surgery: * before lung cancer surgery, * the next day lung cancer surgery, * 3 days after lung cancer surgery. They will be treated in flow cytometry.
Sponsors
Centre Hospitalier Universitaire de Saint Etienne
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patient ≥ 18 years undergoing lung cancer surgery by thoracoscopy
Exclusion criteria
* Patient under guardianship/curatorship * Patient who received of radiotherapy or chemotherapy in the last 6 months * Patient under immunosuppressive treatment or dose of corticoids over 10 mg/day prednisolone or equivalent * Patient with history of malignant blood disease or auto-immune disease * Patient suffering from HIV infection and * Patients with pre-operative infection * Patient with an empiric antibiotic therapy introduced at operating room
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| PD-1, TIM-3, CTLA4 on CD4/CD8 lymphocytes | Days 0, 1, 3 | Correlation between expression of PD-1, TIM-3, CTLA4 on CD4/CD8 lymphocytes and the occurrence of lymphopenia. Measured by blood sample before lung cancer surgery, the next day lung cancer surgery and 3 days after lung cancer surgery. Analyzed by multicolour immunolabelling in flow cytometry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| functionality of CD4/CD8 lymphocytes and production of IF gamma, TNF and IL-2 | Days 0, 1, 3 | Correlation between functionality of CD4/CD8 lymphocytes and production of interferon (IF) gamma, Tumor Necrosis Factor (TNF) and interleukine (IL-2). Measured by blood sample before lung cancer surgery, the next day lung cancer surgery and 3 days after lung cancer surgery. Analyzed by multicolour immunolabelling in flow cytometry |
| pneumonia | Days 3 | To analyse the occurrence of postoperative pneumonia. |
Countries
France
Outcome results
None listed