Nontuberculous Mycobacterium Infection, Whole Genome Sequencing
Conditions
Brief summary
The purpose of the study is to identify the genetic closeness of NTM derived from patients living together and from their surrounding environment and to trace the temporal relationship between them.
Detailed description
After NTM isolation is done by using culture, from patients' sputum/bronchoscopic samples and their environmental samples, whole genome sequencing (WGS) will be conducted for all NTM isolates. Phylogenetic analysis will be performed based on single nucleotide polymorphism (SNP) on each NTM.
Interventions
None listed
Sponsors
Seoul National University Hospital
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* The adult over 18 years of age * The patient who have respiratory symptoms consistent with NTM, such as a chronic cough, sputum, and hemoptysis, who have NTM-positive findings on CT, and who have NTM identification in two or more sputum cultures or in one or more bronchoscopic/tissue culture. (2007 ATS/IDSA guideline) * Pairs of patients living in the same household since diagnosis on NTM lung disease * Consent to a collection of environmental samples from the patient's residence.
Exclusion criteria
* If the patient does not agree or withdraw.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Genetic similarity of NTM(Non-Tuberculous Mycobacterium) | Baseline | Concordance of Single Nucleotide Polymorphisms (SNP) from NTMs derived from patients and their environments |
Countries
South Korea
Contacts
Primary ContactJae-June Yim, MD
Outcome results
None listed