Coronary Heart Disease
Conditions
Brief summary
The objective of this study is to identify relevant signature gene networks of cardiovascular disease in endothelial cells derived from circulating endothelial progenitor cells of individuals with established cardiovascular disease (CVD).
Detailed description
The investigators propose to enroll CVD and healthy subjects between 30 and 60 years of age, matched for age, gender, body mass index, and medication (statin and anti-hypertensive) use. Circulating endothelial progenitor cells will be isolated from blood and cultured in vitro to differentiate into endothelial cells.
Interventions
OTHERisolation of endothelial cells precursors in blood
Circulating endothelial precursor cells will be isolated and then cultured in vitro. RNA will be then isolated.
Sponsors
Massachusetts General Hospital
Tufts University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
30 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
for CVD subjects:: • \>50% stenosis of one or more coronary arteries Inclusion Criteria for Healthy subjects: • absence of coronary artery stenosis
Exclusion criteria
for all subjects: * type 2 diabetes mellitus * chronic kidney disease, * other chronic diseases * dyslipidemia (fasting plasma LDL-C \>160 mg/dL and triglyceride levels \>150 mg/dL)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gene expression | 10 weeks | Gene expression \[ identify relevant signature gene networks of cardiovascular disease in endothelial cells\] |
Countries
United States
Outcome results
None listed