Intrauterine Microbiota During IVF in Patients Affected With Isthmocele.

Endometrial and Isthmic Microbiota During IVF Stimulation in Patients Affected With Isthmocele and Intrauterine Mucus Secretion After C-section.

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03519763

Enrollment

Registered

2018-05-09

Start date

2018-06-13

Completion date

2020-02-24

Last updated

2020-02-25

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Endometrial Diseases

Brief summary

To evaluate the endometrial and isthmic microbiota in patients with isthmocele after C-Section, and if this microbiota is similar or not with better reproductive outcomes.

Detailed description

A total of 45 subjects undergoing in vitro fertilization treatment. Fifteen patients without isthmocele will be included in the control group and 30 patients affected with isthmocele in the other group, divided in 2 subgroups: 15 with 1 previous C-Section, and 15 with 2 or more previous C-Section. Paired samples of endometrial fluid (EF) and isthmic fluid (IF) will be obtained simultaneously the day of egg retrieval. Patients will follow a protocol for all-freezing embryos or oocytes.

Interventions

DIAGNOSTIC_TESTultrasound

All the patients will have a transvaginal ultrasound evaluation during the first visit to the clinic to evaluate the presence or not of isthmocele.

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 45 Years

Healthy volunteers

Yes

Inclusion criteria

1. Patients with isthmocele: * Patients performing IVF treatment with a planned cycle segmentation and consecutive frozen embryo transfer on a hormone replacement therapy (HRT) cycle or natural cycle. * Age between 18 - 45 years all (both inclusive). * One or multiple C-sections. * Isthmocele present during TV scan. * BMI: 18,5 - 35 kg / m2 (both inclusive). 2. Patients without isthmocele: * Patients performing IVF treatment with planned cycle segmentation and who would have embryo transfer with frozen embryos under hormone replacement therapy. * NO Isthmocele present during transvaginal ultrasound. * BMI: 18,5 - 30 kg / m2 (both inclusive).

Exclusion criteria

* Age \< 18 and \> 45 years old. * Previous or active endometritis. * Patients who are IUD carriers for the last 3 months. * Patients who have taken prescribed antibiotics in the last 3 months previous to sample collection.

Design outcomes

Primary

Measure	Time frame	Description
If the intrauterine microbiota profile in patients affected with isthmocele differs from the one found in patients without isthmocele.	1year	patients undergoing in vitro fertilization treatment

Countries

United Arab Emirates

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026