Healthy
Conditions
Brief summary
This is an open-label, non-randomized, single dose, pharmacokinetic study in 36 healthy adult male and female volunteers, including 18 smokers and 18 non-smokers. All subjects will be administered a single inhaled dose of Staccato alprazolam 1 mg via hand-held inhaler. Blood samples will be drawn for pharmacokinetic analysis. Eligible subjects are admitted to Phase 1 unit for up to 48 hours. Subjects will receive a post-study safety phone call 14 days(± 2 days) after dosing.
Interventions
DRUGStaccato Alprazolam 1 mg
alprazolam I mg administered via the Staccato hand-held inhaler device system.
Sponsors
Clinilabs, Inc.
Engage Therapeutics, Inc.
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
All subjects receive a single dose of Staccato Alprazolam 1 mg
Eligibility
Sex/Gender
ALL
Age
21 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* These inclusion criteria (items 1-8) apply to all subjects, smokers and non-smokers: I. Healthy male and female subjects between the ages of21 to 50 years, inclusive. 2\. Body mass index (BMI) \>/= 18 and \</= 30 kg/m2, inclusive. 3\. Able to speak, read, and understand English and are willing and able to provide written informed consent on an IRB-approved form prior to the initiation of any study procedures. 4\. Willing and able to be confined to a clinical research facility for up to 48 hours (including 2 nights) and comply with the study schedule and study requirements. 5\. Normal spirometry at screening as demonstrated by FEVI +/- 80% of predicted and FVC +/- 80% of predicted. 6\. Adequate veins, as assessed by the Investigator or Investigator's designee, that are suitable for the required number and frequency of PK blood draws in this study. 7\. In otherwise good general health as determined by a complete medical history, physical examination, 12-lead electrocardiogram (ECG), blood chemistry profile, hematology, serology (HIV-l/2Ab, HBsAg, HCV Ab), and urinalysis at screening. 8\. Female participants (if of child-bearing potential and sexually active) and male participants (if sexually active with a partner of child-bearing potential) who agree to use a medically acceptable and effective birth control method throughout the study and for one week following the end of the study. Smokers must meet the following inclusion criteria (items 9-10): 9\. A history of smoking\> 15 cigarettes/day currently and for at least the last 2 years. 10\. Serum cotinine +/-500 ng/mL at Screening Visit I. Nonsmokers must meet the following inclusion criteria (items 11-12): 11\. A history of never smoking\> 5 cigarettes/day and not smoking at all for at least the last 2 years prior to Visit I. 12\. Serum cotinine :o 40 ng/mL at Screening Visit I and a negative urine cotinine test at Visit 2 Day-I.
Exclusion criteria
1. Treatment with an investigational drug within 30 days ( or within 5 half-lives of the investigational drug, if\>30 days) prior to Visit I. 2. Significant hepatic, renal, gastroenterologic, cardiovascular endocrine, neurologic (including history of seizures or stroke), or hematologic disease. 3. Any acute illness in the 5 days prior to Visit 2. 4. Upper respiratory tract infection within 6 weeks of Visit 2 or bronchitis or pneumonia within 6 months of Visit 2. 5. Use of a bronchodilator for the treatment of wheezing within 12 months of Visit I. 6. Diagnosis of an active or chronic pulmonary disease. 7. Lung resection or other pulmonary surgery within 12 months of Visit I. 8. A history of allergy or intolerance to alprazolam. 9. Use of any other prescription or nonprescription medication--with the exception of acetaminophen, ibuprofen or ongoing doses of oral contraceptives or vitamins--within 5 days prior to study drug administration. 10. A history within the past 2 years of drug or alcohol dependence or abuse. 11. Positive test for alcohol or a positive urine screen for drugs of abuse at screening 12. A positive HIV test. 13. Breastfeeding or a positive pregnancy test at screening (female subjects). 14. Clinically significant ECG abnormality. 15. Hypotension or hypertension, at screening or baseline. 16. Poor veins in the opinion of the Investigator or the Investigator's designee such that the participant is judged to likely have difficult venipuncture during the study. 17. Any other disease or condition, by history, physical examination, or laboratory abnormalities that in the investigator's opinion, would present undue risk to the subject, or may confound the interpretation of study results. 18. Participation in another clinical trial within 2 months of the beginning of the present study, or blood donation or comparable blood loss (\>350mL) within 2 months before the present study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Pharmacokinetics (PK) : Maximum observer drug concentration (Cmax) | Sequential time points : pre-dose, and at 2, 5, 10 and 30 minutes, and at 1, 2, 4, 6, 12, 24 and 36 hours post-dose. | Cmax of alprazolam in smokers compared to non-smokers |
| PK: Area under the concentration versus time curve (AUC)from Zero to Infinity (AUC[0-∞] | Sequential time points : pre-dose, and at 2, 5, 10 and 30 minutes, and at 1, 2, 4, 6, 12, 24 and 36 hours post-dose. | AUC\[0-∞\] of alprazolam in smokers compared to non-smokers |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| PK;: time after administration of a drug when the maximum plasma concentration is reached (Tmax) | Sequential time points : pre-dose, and at 2, 5, 10 and 30 minutes, and at 1, 2, 4, 6, 12, 24 and 36 hours post-dose. | Tmax of alprazolam in smokers compared to non-smokers |
| PK: half-life (t1/2) | Sequential time points : pre-dose, and at 2, 5, 10 and 30 minutes, and at 1, 2, 4, 6, 12, 24 and 36 hours post-dose. | t1/2 of alprazolam in smokers compared to non-smokers |
Countries
United States
Outcome results
None listed