Metabolic Disturbance
Conditions
Keywords
Gut microbiota, Hepatic function, Obesity, Exercise
Brief summary
This project offers the opportunity to obtain detailed measurements on the health of body composition, metabolic health, and intestinal microbial diversity in overweight adults; moreover to provide information about the effects of physical exercise on them. The aim of the research is to advance in the understanding of the mechanisms induced by physical exercise, which includes the measurement of nutritional parameters, clinical and biochemical biomarkers of hepatic/cardiometabolic health, as well as the application of differential proteomics technologies by means of Arrays of cytokines and intestinal microbiome, making this study a novel and pioneer in sensitive areas of primary health care in Latin America.
Interventions
BEHAVIORALHIIT group
Physical Training
Sponsors
Universidad de Santiago de Chile
Universidad Pública de Navarra
Universidad del Rosario
NavarraBiomed Biomedical Research Center
Universidad Santo Tomas
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Sedentary males * BMI\>25 kg/m2, on a stable body weight for the last 3 months
Exclusion criteria
* Acute or chronic illness * Use of antibiotics the past 2 months * Smoking
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gut microbiota | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline in the gut microbiota composition measured by amplicon sequencing |
| Plasma metabolomic profile | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline in the metabolomic profile will be also evaluated by a HPLC-MS approach |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Insulin | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline will be also evaluated by a ELISA |
| Body composition | Baseline, and 6 weeks immediately after the interventions ends | Differences in body weight (kilograms) measured by DXA |
| Glycated hemoglobin | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline will be also evaluated by a ELISA |
| Plasma hepatokines | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline in the Chemerin will be measured using ELISA kits |
| Physical fitness | Baseline, and 6 weeks immediately after the interventions ends | Differences in Muscular strength (handgrip test) in kg |
| Energy substrate oxidation | Baseline, and 6 weeks immediately after the interventions ends | Fat and carbohydrate oxidation in the fasted and postprandial state measured before and after the interventions using indirect calorimetry (K5 cosmed) |
| Resting Metabolic Rate | Baseline, and 6 weeks immediately after the interventions ends | Resting metabolic rate was measured before and after the interventions (K5 Cosmed) |
| Bioinflammatory blood markers | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline measure of plasma IL-1 concentrations measured ELISA |
| Glucose | Baseline, and 6 weeks immediately after the interventions ends | Change from Baseline will be also evaluated by a spectroscopy |
Countries
Colombia
Outcome results
None listed