Chronic Disease, Intestinal Disease, Parasitic
Conditions
Keywords
Per os invasions, Giardiasis, Toxoplasmosis, Taeniasis, Cryptosporidiosis, Trichinosis, Ascariasis, Echinococcosis
Brief summary
The goal of the proposed study is to assess the prevalence of intestinal parasitic diseases in adults and children. These invasions are included to dirty hand diseases (per os invasions), therefore it applies mainly preschool children.
Detailed description
It is estimated that 75% of the world's population is infected with parasites and although parasitic diseases are mostly observed in the poor tropical and subtropical countries, there are also many diseases caused by parasites in Poland. In Poland, particular attention is paid to the following parasitic diseases closely related to the digestive system: giardiasis, taeniasis (including cysticercosis), ascariasis, cryptosporidiosis, toxoplasmosis, trichinosis, echinococcosis. Symptoms of such invasions may include: allergic rhinitis of unknown origin, chronic fatigue or food intolerance. Moreover, many of them are asymptotic, therefore the study group will consist of both healthy people and individuals with undiagnosed diseases or/and chronic symptoms.
Interventions
DIAGNOSTIC_TESTTests for diagnosis of intestinal parasitic diseases
Feces testing: 1. fresh fecal samples will be used to preparing direct (saline and iodine wet) mounts using supplementary techniques (flotation, sedimentation), as well as temporary mounts (hematoxylin and trichrome staining). Other technique of fresh fecal samples examination will be ParaSys™ 2. A non-preserved stool samples will be stored below -20°C. Defrosted fecal specimen will be subjected to enzyme-linked immunosorbent assays (ELISA) - Giardia antigen test (coproantigens)
Sponsors
Nicolaus Copernicus University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
3 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* healthy and ailing individuals * men and females * adults and children
Exclusion criteria
* the participants were not be: incapacitated persons, soldiers, prisoners and persons dependent in any way from the investigators
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Intestinal parasites | 1 day (single evaluation of current samples) | The fecal samples will be assessed qualitavely in terms of life forms of parasites present in human intestine, i.e.: eggs and larvas of flukes, tapeworms and nematodes, as well as protozoa trophozoites and cysts. |
| Giardia antigen test | 1 day (single evalutaion of all samples through study completion) | Stool test for Giardia lamblia antigen (ELISA kit) |
Countries
Poland
Outcome results
None listed