Healthy Men and Women
Conditions
Keywords
metabolism, metabolome, physical activity, hypoxia
Brief summary
The aim of this study is to systematically Analyse the metabolome in capillary blood of healthy men and women at rest and during defined standardized physical activity under normobaric normoxia or hypoxia with a specific focus on metabolites and metabolic pattern related to energy metabolism. The metabolome analyses in capillary blood could give us also or more detailed insight into erythrocyte metabolic physiology because it is known for a longer time that the primary metabolism of the erythrocyte reflects its Oxygen Saturation via the glycolytic metabolites / intermediates pattern.
Interventions
OTHERrunning on a treadmill under normoxia or hypoxia
* 30 min running (60% VO2max) on a treadmill under hypoxia (O2: 14%) * 30 min running (60% VO2max) on a treadmill under normoxia (O2: 20%)
Sponsors
Charite University, Berlin, Germany
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy volunteers * written informed consent
Exclusion criteria
* known cardio-vascular, lung, metabolic, endocrine, hematologic, kidney or neurologic diseases * pregnancy, lactating period * unability to understand study content and aims and to give written informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in capillary lactate/pyruvate Ratio (dimension-free) | t = 0 min (before exercise); t = 10, 20 and 30 min during exercise; t = 50 and 60 min during recovery | marker for the ratio between anaerobic / aerobic glycolysis |
Countries
Germany
Outcome results
None listed