Vegetable Signature Documentation With Metabolomic Technique
Conditions
Keywords
vegetable metabolite signature, human serum/urinary metabolite, liquid chromatography mass spectrometry
Brief summary
The purpose of this study is to perform dietary intervention studies on a variety of vegetables.
Detailed description
Plant-based foods have always been an important component of a healthy diet. High intake of plant foods may not only maintain normal bodily functions through the provision of dietary fiber, vitamins, and minerals but also relieve symptoms and prevent diseases. Many studies have pointed out that high intake of plant foods may reduce the incidence of non-communicable disease. Past research investigating the effect of phytochemicals has usually been limited to single substances or single foods. However, a number of researchers have pointed out that phytonutrients and dietary components are likely to act synergistically.Health effects and mechanisms of phytonutrients in combination have not been carefully studied in population settings. The technology of metabolomics has enabled the simultaneous measurement of hundreds of small molecules in biological samples. Exploring the change of metabolites after feeding can not only help us understand the role of plant foods, but may also provide opportunities to assess plant food intake levels.
Interventions
A standardized diet without any vegetables was provided.
A standardized diet with 200g spinach was provided.
A standardized diet with 200g celery was provided.
A standardized diet with 200g onion was provided.
A standardized diet with 200g of mixed vegetables (spinach, celery, and onion) was provided.
Sponsors
Study design
Eligibility
Inclusion criteria
* BMI 18.5\ 30 kg/m2 * non-smoker * non-alcoholic
Exclusion criteria
* Unwilling to stop taking the dietary supplements that may affect the experiment result. * Unwilling to take the provided meal during the designated period. * Taking hypertension or diabetes drugs. * Diagnosed immune diseases (such as allergic diseases, autoimmune diseases, etc.), liver diseases, metabolic diseases (such as hyperthyroidism or hypothyroidism), or other not suitable for the dietary study. * Diagnosed cancer or other severe diseases. * Suffered from urinary tract infection or took antibiotic in the past three months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Intensities of multiple serum metabolites | Baseline (fasting state before breakfast at 7:30AM) | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. |
| Intensities of multiple urinary metabolites | Baseline (overnight urine sample collected at 7:30AM) | The outcome measure consists of intensities of multiple urinary metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. |