Effect of Spinal or Sevoflurane Anesthesia on Neutrophil Activation During Knee Surgery With Pneumatic Tourniquet

Effect of Spinal or Sevoflurane Anesthesia on Neutrophil Activation After Tourniquet Induced Ischemia-reperfusion in Knee Surgery

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03470363

Enrollment

Registered

2018-03-19

Start date

2016-01-01

Completion date

2018-03-01

Last updated

2020-03-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Knee Surgery With Tourniquet

Keywords

total knee arthroplasty, tourniquet, sevoflurane, spinal anesthesia, myeloperoxidase, elastase, neutrophil

Brief summary

Detailed description

Aim of study : Assessement of neutrophil activation during ischemia reperfusion associated with tourniquet use for total knee arthroplasty. This activation was compared in a group of patient scheduled for spinal anesthesia and for sevoflurane anesthesia This activation was assessed by plasma myeloperoxidase and elastase measurements in four consecutive blood samples during and after surgery (before surgical incision and 1, 3, 24 hours following tourniquet release.

Interventions

DRUGSevoflurane Volatile Liquid

DRUGBupivacaine / SUFentanil

Study design

Allocation

NON_RANDOMIZED

Intervention model

PARALLEL

Primary purpose

SCREENING

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* Total knee arthroplasty under spinal or sevoflurane anesthesia

Exclusion criteria

* American Society of Anesthesiologists class 3 and 4 * vascular disease of the lower limbs * cardiac or respiratory disease * immune disorder

Design outcomes

Primary

Measure	Time frame	Description
Changes in plasmatic concentration of myeloperoxidase and elastase	Before surgical incision and one hour after tourniquet release	Biological measurement of changes in plasmatic concentration of myeloperoxidase and elastase from preoperative values to one hour after tourniquet release.

Secondary

Measure	Time frame	Description
Changes in plasmatic concentration of endothelial biomarkers	Before surgical incision and one hour after tourniquet release	Biological measurement of changes in plasmatic concentration of syndecan-1, soluble thrombomodulin, soluble E-selectin and VEGF from preoperative values to one hour after tourniquet release.

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026