Healthy Subjects, Hyperalgesia, Pain Response
Conditions
Keywords
NGF-induced pain, Ischemia-induced pain, Hyperalgesia, NGF pain model
Brief summary
The purpose of this study is to investigate pain evoked responses and facilitation of NGF-induced mechanical muscle hyperalgesia over time following an acute exercised-induced ischemic condition in a NGF-sensitized muscle.
Detailed description
Assessing evoked pain responses and muscle hyperalgesia following contractions during acute ischemia in a NGF-sensitized muscle may clarify whether an interaction between NGF sensitization and acidic stimulation exist. It is hypothesized that acute ischemic exercise with the NGF-sensitized TA muscle, in contrast to ischemic exercise in a non-sensitized would: 1) potentiate pain-evoked responses, and 2) facilitate NGF-induced muscle hyperalgesia.
Interventions
DRUGNGF
Intramuscular injection
DRUGIsotonic saline
Intramuscular injection
Sponsors
Aalborg University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy and pain free volunteers
Exclusion criteria
* Pregnancy * Drug addiction defined as the use of cannabis, opioids or other drugs * Previous neurologic, mental illnesses, or psychiatric diseases. * Past history of chronic pain in the musculoskeletal system (muscle, joint, cartilage, connective tissue) * Participation in other pain trials throughout the study period * Lack of ability to cooperate * Taking any analgesic 24 hours before the injections * Performing any strenuous leg exercise through out the study period causing sore muscles
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle sensitivity | Change from baseline at 1 week | Pressure pain thresholds (PPTs) are assessed over the non-dominant tibialis anterior muscle using a handhold pressure algometer. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Ischemic-induced pain intensity | Assessed right after the dorsiflexions have been performed | Subjects perform 45 dorsiflexions with their non-dominant leg while a cuff is mounted over the knee to occlude the blodflow from the muscle (ischemia) Subjects subsequently rate their perceived pain intensity verbally on a numeric rating scale (NRS) |
| Functional muscle pain | Change from baseline at 1 week | Subjects evaluate their muscle pain during movement using a Likert Scale for lower limp |
| Muscle pain diary | Change from baseline at 1 week | Subjects evaluate their muscle pain during movement using a Likert Scale for lower limp and perceived pain intensity at rest on a numeric rating scale (NRS). The diary is filled out at home on days between sessions |
Countries
Denmark
Outcome results
None listed