Cardiovascular Health, Identify Oral Bacteria Responsible for Nitrate Reduction
Conditions
Brief summary
This study evaluates whether dietary manipulation of the oral microbiota modulates cardiovascular health through effects on nitric oxide bioavailability.
Detailed description
Nitric oxide is an important molecule for human health because it controls blood flow and blood pressure. With aging, our ability to make nitric oxide gets worse and our blood pressure tends to increase. We can help the body produce more nitric oxide by supplementing the diet with vegetables, such as beetroot juice, which contains high amounts of nitrate. This has been shown to improve blood flow and blood pressure. The bacteria inside our mouths play an important role in helping convert the ingested nitrate to nitric oxide. The reason for this study is to find out whether there are differences in oral bacteria between young and older adults, and how the oral bacterial community and blood flow and blood pressure might change when diet is supplemented with natural, nitrate-rich beetroot juice and when we use antibacterial mouthwash. This study will help us understand how the bacteria inside our mouths might be useful for keeping us healthy.
Interventions
DIETARY_SUPPLEMENTNitrate-rich beetroot juice
concentrated beetroot juice
DIETARY_SUPPLEMENTNitrate-depleted beetroot juice
concentrated beetroot juice
OTHERMouthwash
2 x 10 mL antibacterial mouthwash per day for 14 days
Sponsors
University of Exeter
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy young (18-30 yr) and older adults (65-80 yr) * Male and female * Willing to undergo the experiments as detailed in the informed consent documentation * Willing and capable of providing written, informed consent.
Exclusion criteria
* Individuals receiving medication for pulmonary, cardiovascular, or metabolic conditions, ulcerative colitis or renal disease * Having an active oral disease or dentures * Having resting BP \>140/90 mmHg * Having used antibiotics within 3 months * Lacking willingness or capacity to give informed consent.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Quantity of nitrate -reducing bacteria | 14 days | Change in total quantity of nitrate-reducing bacteria as measured by sequencing bacterial 16S rRNA genes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Nitric oxide bioavailability - salivary nitrite | 14 days | Change in nitrite concentration of saliva as measured by ozone-based chemiluminescence |
| Nitric oxide bioavailability - plasma nitrate | 14 days | Change in nitrate concentration of plasma as measured by ozone-based chemiluminescence |
| Nitric oxide bioavailability - plasma nitrite | 14 days | Change in nitrite concentration of plasma as measured by ozone-based chemiluminescence |
| Blood pressure | 14 days | Change in systolic and diastolic BP |
| Flow mediated dilation | 14 days | Change in flow mediated dilation |
| Nitric oxide bioavailability - salivary nitrate | 14 days | Change in nitrate concentration of saliva as measured by ozone-based chemiluminescence |
Countries
United Kingdom
Outcome results
None listed