Embryo Culture
Conditions
Brief summary
The aim of this study is to analyze whether there is difference in the effect of single embryo culture and group culture in preimplantation genetic screening (PGS) cycle, and provide reference for the feasibility of the clinical application of medium-based noninvasive PGD. This prospective study prepares for more than 1,000 normal fertilized zygotes in each group over one year period. For the recruited patients, all normal fertilized zygotes were randomly allocated into two groups: cultured individually in 25 ul media or 2-3 embryos cultured together in 50 ul media. The blastocyst formation rates, aneuploid rates, clinical pregnancy rates, implantation rates and miscarriage rates will be compared between the two groups.
Interventions
OTHERembryo culture
Single embryo culture means only one embryo is cultured in one drop, and the group culture refers to culture of 2-5 embryos in one drop. The main advantage of group culture may be secreting some paracrine cytokines by embryos and benefit each other; and the possible disadvantage of group culture is that embryo secreting of some harmful substances which accumulated to a certain extent will damage the developmental potential of embryos. The advantages and disadvantages of single embryo culture are contrary to group culture.
Sponsors
Reproductive & Genetic Hospital of CITIC-Xiangya
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* patients treated by preimplantation genetic diagnostic / screening cycles
Exclusion criteria
* number of normal fertilization zygotes is less than 6
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| high quality blastocyst formation rate | 6 days after fertilization | number of blastocysts with high quality divided by number of zygotes with normal fertilization |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| embryo euploid rate | about one month after blastocyst biopsy | number of euploid blastocysts divided by all blastocysts detected |
| embryo implantation rate | about three to six months after blastocyst biopsy | number of gestational sacs divided by number of embryo transferred |
Countries
China
Contacts
Primary ContactShuoping Zhang, Doc
Backup ContactLu Tan, Mrs
Outcome results
None listed