Healthy
Conditions
Keywords
Sleep, Blood Sugar
Brief summary
This study plans to learn more about how sleep loss impacts the way the body responds to sugar. This work will have important implications for development of treatments and countermeasures for people who are not able to get enough sleep for various reasons.
Detailed description
Participants will stay in the hospital for 6 days and will not be able to leave. Sleep habits will be measured for 7 days before the study. A research diet that is designed to maintain weight will be provided for 3 days before the study and during the study. Multiple tests will be performed, including metabolic testing and muscle and fat biopsies, both when participants first arrive and have had normal sleep and again after 4 nights of only 5 hours time in bed. The visit takes place at the Clinical Translational Research Center (CTRC) at the University of Colorado Denver, Anschutz Medical campus. It will take approximately 2 weeks to complete this study.
Interventions
BEHAVIORALInsufficient sleep
Four days of insufficient sleep
Sponsors
University of Colorado, Denver
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
The project is a within-subject consecutive design examining 18 healthy individuals after baseline and after 4 nights of insufficient sleep.
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
1. Healthy, lean participants. 2. No regular physical activity (\<20 minutes/day). No exercise will be allowed for 3 days prior to study start. 3. Habitual sleep duration between 7-9.25h. Subjects must also be willing and able to keep a 9h time-in-bed schedule for 1 week prior to study admission. 4. Potential subjects must have lived at Denver altitude or higher for at least 3 months to inpatient study.
Exclusion criteria
1. Current or history of any clinically significant medical, psychiatric, or sleep disorder. 2. Use or history of any drugs, medications, supplements, caffeine, and alcohol. 3. Current or history of shiftwork in six months prior to laboratory study. 4. Travel more than one time zone in three weeks prior to laboratory study. 5. Blood donation in the 30 days prior to inpatient study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in insulin sensitivity | One week | Insulin sensitivity measured by hyperinsulinemic euglycemic clamp. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in macrovascular function | One week | Macrovascular function will be assessed with flow-mediated dilation |
| Changes in microvascular function | One week | Macrovascular function will be assessed with EndoPAT |
| Changes in cognitive abilities | One week | Cognitive computer tests performed during study |
| Changes in circadian rhythms | One week | Salivary dim-light melatonin offset will be assessed |
| Changes in metabolic tissue function | One week | Tissue biopsies will be performed |
Countries
United States
Outcome results
None listed