Femoral Neck Fractures
Conditions
Brief summary
RATIONALE: Collecting and storing bone samples from patients with osteoporotic and nonosteoporotic fracture to quantitatively analyze the differentially expressed proteins to further explain the relationship between bone iron content and bone mineral density (BMD). PURPOSE: This research study is looking at changes of iron content and differentially expressed proteins in bone samples from patients with osteoporotic and nonosteoporotic. fracture.
Detailed description
OBJECTIVES: 1. Establish the iron content in bone specimen in patients with hip fracture. 2. Explore the correlation of BMD and differentially expressed proteins. OUTLINE: 1. Collect human bone specimens from patients with osteoporotic and nonosteoporotic Fracture. 2. Provide a repository for storage of tissue and make these specimens available for approved projects by laboratory-based investigators. 3. Collect clinical data on these patients including bone mineral density. 4. To examine the bone iron content using ICP/MS. 5. Investigate the differentially expressed proteins through quantitative analysing the global proteome of osteoporotic and nonosteoporotic fracture bone specimens.
Interventions
PROCEDUREtotal hip arthroplasty
Bone samples from Hip fracture were collected from the femoral head which has been replaced
Sponsors
Second Affiliated Hospital of Soochow University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
FEMALE
Age
55 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
1. Ages Eligible for Study: 55-90 Years 2. Genders Eligible for Study: Woman 3. Accepts Healthy Volunteers: NO 4. Study Population: Female patients with hip fracture.
Exclusion criteria
1. Malignancy or benign ovarian cysts 2. Known chronic or systemic diseases 3. Hormone therapy in the previous 3 months 4. Bone metabolism and drug therapy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| the iron content in human bone samples | 3 month | Bone samples were preprocess and decomposed by microwave.Icp-ms is used for quantitative detection of samples. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The value of bone mineral density | one week | Bone mineral density was measured by double energy X-ray absorptiometer (DXA) in a week before surgery. |
| Quantitative Analysis of Global Proteome in bone samples | 5 month | Find the differentially expressed proteins in nonosteoporotic or osteoporotic bone sample through using an integrated approach involving TMT labeling and LC-MS/MS to quantify the dynamic changes of the whole proteome of Human Species. |
Countries
China
Contacts
Primary ContactYoujia Xu, PhD
Backup ContactFan Yang, MSc
Outcome results
None listed