Lung Cancer
Conditions
Brief summary
The study will investigate the role of 1,3-butadiene metabolic activation and deactivation in lung cancer risk among various ethnic/racial groups. This project will require urine samples from smokers and nonsmokers from the three ethnic/racial groups recruited by the Clinical and Biomarker Core for the analysis of 1,3-butadiene DNA adducts. Data on nicotine intake (urinary TNE) in these subjects as well as in 400 lung cancer cases and 400 controls from Project 1 will be also required for this project.
Interventions
DIAGNOSTIC_TESTUrine Sample
One to two home visits where tobacco use and medical history and biological samples will be collected including blood, buccal cells and urine
Sponsors
National Institutes of Health (NIH)
Masonic Cancer Center, University of Minnesota
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
21 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* One of the three targeted ethnic groups: * Japanese American - two parents of Japanese descent * Non-Hispanic Whites - two parents of non-Hispanic white descent * Native Hawaiians will include individuals with at least one parent of Hawaiian descent; * Smoke 5 cigarettes per day over the past three months; * \>21 years of age; * Consumes 14 or fewer drinks of alcohol per week; * Generally stable and good health (determined by review of medical history); * Able to provide written voluntary consent before performance of any study related procedure.
Exclusion criteria
* Current use of other nicotine containing products for \> 4 times per month (and no use of any nicotine-containing products except cigarettes for 2 weeks prior to their study visits); * Acute or uncontrolled medical or psychiatric conditions; * Currently taking any medications that affect relevant metabolic enzymes or anti- inflammatory medications such as ibuprofen (this will be reviewed by study investigators on a case-by-case basis); * Active infection (e.g., influenza, cold, respiratory infection, sinus infection) at the time of the visit; * Pregnant or nursing or planning on becoming pregnant during the study; * Unable to read and understand English.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Butadiene-DNA adducts in different ethnic groups | Day 1 & 2 | Investigate ethnic differences in butadiene-DNA adducts in smokers belonging to different ethnic groups. |
| Butadiene-DNA adduct load in smokers with lung cancer | Day 1 & 2 | Investigate the association between butadiene-DNA adduct load and lung cancer development in smokers. |
| Polymorphisms of carnicogen metabolizing genes | Day 1 & 2 | Examine how polymorphisms of carcinogen metabolizing genes influence metabolic inactivation, DNA adduct formation/repair, and toxicity of butadiene-derived epoxides. |
Outcome results
None listed