Gut Microbiota, Infection, Spleen Injury
Conditions
Keywords
Gut microbiota, Splenectomy, Infection, Immune, Cytokines
Brief summary
Studies has shown an increasingly infection rate after splenectomy, and there is a potential correlation between microbiota and immune system. investigators suppose that increasingly infection can be associated with the alteration composition of the gut microbiota after splenectomy. It's investigators' aim to discover if any difference of gut microbiota is exist in patients who suffer from traumatic splenectomy compared with normal people, ultimately aim toreduce and mitigation infection rate through controlling gut microbiota.
Detailed description
The spleen is crucial in regulating immune homoeostasis through its ability to link innate and adaptive immunity and to protect against infections. Asplenia refers to the absence of the spleen, a disorder that is rarely congenital and is more frequently as a result of surgery. Splenic hypofunction, as a result of asplenia can lead a series of changes in body systems. Recent study has show an increasingly infection rate after splenectomy including abdominal infection, pulmonary infection and cranial cavity infection. The gastrointestinal tract plays host to a diverse and metabolically complex community of microorganisms. Recent literature suggests that organisms in the gastrointestinal tract, referred to collectively as gut microbiota, play an indispensable role in the maintenance of host's homeostasis. Study has proved a potential correlation between microbiota and immune system. Lymphocyte, in either peripheral circulation or mesenteric lymph node, altered can lead to an composition change in microbiota. Investigators suppose that this phenomenon can be associated with the alteration of the resident commensal microenvironment after splenectomy compared to commensal communities. It's investigators' aim to discover if any difference of gut microbiota is exist in patients who suffer from traumatic splenectomy compared with normal people, ultimately aim toreduce and mitigation infection rate through controlling gut microbiota.
Interventions
PROCEDUREsplenectomy
Traumatic patients who performed total splenectomy
Sponsors
First Affiliated Hospital of Harbin Medical University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* All the patients underwent an total splenectomy in the First Affiliated Hospital of Harbin Medical University from January 1st, 2015 to May 1st, 2017. Each participant provided a fresh stool sample in hospital when following-up.
Exclusion criteria
* Use antibiotics and probiotics 3 mouth before samples collection. * Other severe abdomen injury * Underwent abdomen organ resection surgery * Other digestive system disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Transcriptional changes in gut microbiota | Baseline, 6 months after surgery, 12 months after surgery, 24 months after surgery | 16S rRNA gene sequencing will be performed with stander procedure |
| Transcriptional changes in plasma LPS levels | Baseline, 6 months after surgery, 12 months after surgery, 24 months after surgery | Plasma LPS level will be measured by ELISA |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Fecal SIgA | Baseline, 6 months after surgery, 12 months after surgery, 24 months after surgery | Fecal SIgA antibody will be measured by ELISA |
| Fecal calprotectin | Baseline, 6 months after surgery, 12 months after surgery, 24 months after surgery | Fecal SIgA antibody will be measured by ELISA |
| Plasma DAO | Baseline, 6 months after surgery, 12 months after surgery, 24 months after surgery | Plasma DAO antibody will be measured by ELISA |
| Plasma D-Lac | Baseline, 6 months after surgery, 12 months after surgery, 24 months after surgery | Plasma D-Lac antibody will be measured by ELISA |
Countries
China
Contacts
Primary ContactWei Yunwei
Outcome results
None listed