Intestinal Polyps
Conditions
Keywords
phytoestrogens, isoflavones, adenomatous polyps, microbiome
Brief summary
INTRODUCTION: The data obtained by experimental studies about the influence of phytoestrogens on colorectal cancer (CRC) have been very promising. On the other hand, clinical trials have produced conflicting results. The literature suggests that some subclasses of phytoestrogens may have protective effects against CRC and colon adenomas, but most of these results come from population studies based on the dietary intake of phytoestrogens. On these premises, it is possible to hypothesize that the variability of the data reported in the literature may be due to the fact that the real absorption of phytoestrogens (by assessing their concentration in the serum or urine) and/or the ability of the single individual of producing equol was not evaluated. PURPOSE: In the present study, the association between the phytoestrogens intake and the prevalence of colon adenomas was evaluated not only on the basis of the simply dietary intake but also on the measurement their intestinal absorption. Moreover, a specific evaluation of equol production by the intestinal flora was performed. Finally, intestinal bacteria involved in equol production were evaluated.
Detailed description
MATERIALS AND METHODS: Patients with sporadic colon adenomas were enrolled in group I (case) and patients without sporadic colorectal adenomas matched for sex, age and BMI were enrolled in group II (control). All participants underwent the following evaluations: BMI, dietary history (for quantitative and qualitative analysis of dietary habits), quantitative analysis of phytoestrogens (by dietary questionnaires), medications (chronic assumption of aspirin at low doses), characteristics of the polyps (for a calculation the cancer risk), analysis of urinary excretion of phytoestrogens \[by high pressure liquid chromatography (HPLC)\] and intestinal flora \[by mass spectrometry with Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) technology\]. At the enrolment, patients will be invited to assume a standard quantitative of phytoestrogens at 8:00 a.am. and collect both a fecal sample and the urine of the following 24 hrs.
Interventions
None listed
Sponsors
University of Bari
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
50 Years to 75 Years
Healthy volunteers
No
Inclusion criteria
* subjects of both sexes aged between 50 and 75 years * colonoscopy within 3 months from the study evaluation
Exclusion criteria
* age \< 50 and \> 75 years * previous diagnosis of colon cancer or inflammatory bowel diseases (IBD) * Hereditary intestinal tumors (FAP, HNPCC, ...) * ongoing infections * intake, in the last 4 weeks, of drugs that alter the intestinal bacterial flora * creatinine clearance below 60 ml/min * liver failure
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Phytoestrogen absorption | one day | It will be evaluated on the basis of their 24h urine excretion |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Intestinal microbiota | one day | It will be evaluated on fecal samples |
Countries
Italy
Outcome results
None listed