Osteo Arthritis Knee
Conditions
Keywords
exercise, cytokines, collagen
Brief summary
This study will examine the effect of exercise on the turnover of collagen in articular cartilage and the content of cytokines in the synovial fluid from human adults with osteoarthritis.
Detailed description
By using stabile isotope labelling, this study will track the formation and destination of newly formed cartilage collagen in adult humans scheduled for knee replacement surgery due to osteoarthritis. The collagen formation as well as the effect on the cytokine content of the synovial fluid will be examined both at rest and in response to 4 weeks of exercise/training. Overall the project aims to improve the basic understanding of cartilage development and function in relation to osteoarthritis prevention.
Interventions
BEHAVIORALExercise.
Moderate to heavy intensity resistance exercise consisting of leg press.
Sponsors
Bispebjerg Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
40 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
* Primary osteoarthritis of the knee scheduled for knee replacement surgery * Must be able to perform physical exercise * Age 40-90 years old * BMI 18.5-40 kg/m\^2 * None smoker for the last 6 weeks
Exclusion criteria
* Regular intake of: * non steroid anti-inflammatory drugs (NSAID) * corticoid-steroids * disease modifying anti-rheumatic drugs (DMARD) * cytostatic incl. chemotherapy * immune suppressive medicine * Inflammatory diseases or cancer * Recent trauma to the knee (\< 3 months) * Previous knee surgery * Regular strenuous exercise ( \> 1 pr. month) * Previous exposure to tracers (e.g. from another study)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Collagen synthesis in articular cartilage | End of study at 4 weeks | By using incorporation of stabile isotope tracers measured by gas chromatography, the concentration of newly formed collagen will be measured. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cytokine and enzymes concentration in blood | At the beginning and at the end of the study at 4 weeks | By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in the blood will be measured. |
| Gene expression | End of study at 4 weeks | By using real time reverse transcriptase polymerase chain reaction (RT-PCR) the concentration of messenger ribonucleic acid (mRNA) in cartilage tissue will be measured. |
| Cytokine and enzymes concentration in synovial fluid | End of study at 4 weeks | By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in synovial fluid will be measured. |
| Body mass index (BMI) | At the beginning of the study. | BMI measured as the total body weight divided by the height squared (kg/m\^2) |
| Age | At the beginning of the study. | The age measured in years |
| Strength | At the beginning and at the end of the study at 4 weeks | By calculating the total volume of weight lifted (kg) the strength will be measured. |
Countries
Denmark
Outcome results
None listed