Carotid Stenosis, Ischemia Reperfusion Injury
Conditions
Keywords
Matrix metalloproteinase, Carotid artery stenting, Carotid endarterectomy
Brief summary
The aim was the comparison of the perioperative time courses of matrix metalloproteinase-9 (MMP-9) and its inhibitor (TIMP-1) during elective carotid artery stenting (CAS). The investigators used a matched, historical carotid endarteriectomy group as controls. Blood samples at four time points: T1: preoperative; T2: 60 minutes after stent insertion; T3: first postoperative morning; and T4: third postoperative morning. Plasma was isolated from heparin anticoagulated blood samples by low speed centrifugation at 4 °C, and stored at -80 °C until analyzed in a single batch at the end of the study. Plasma concentrations of MMP-9 and TIMP-1 were expressed as ng/ml.
Interventions
PROCEDURECarotid artery stenting
Patient undergo elective carotid artery stenting. In this patient group blood sampling was performed at three time points: T1, at the time of the insertion of the arterial line; T2, 60 min after stent insertion; and T3: the first postoperative morning.
PROCEDURECarotid endarterectomy
Patient undergo elective carotid endarterectomy. Group samples were collected at the following four time points: T1, at the time of the insertion of the arterial line; T2, 60 min after cross-clamp release; T3, the first postoperative morning; and T4, the third postoperative morning. A matched subgroup of patients from the previously obtained data served as a historical control.
Sponsors
University of Pecs
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
elective carotid artery surgery
Exclusion criteria
Malignant diseases, inflammatory and systemic autoimmune disorders, psychiatric disorders and previous debilitating stroke
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma MMP-9 concentrations | 4 days | Plasma is isolated from heparin-anticoagulated blood samples by low speed centrifugation at 4 °C. MMP-9 levels were measured with quantitative sandwich enzyme-linked immunosorbent assay. |
| Plasma TIMP-1 concentrations | 4 days | Plasma is isolated from heparin-anticoagulated blood samples by low speed centrifugation at 4 °C. TIMP-1 levels were measured with quantitative sandwich enzyme-linked immunosorbent assay. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| MMP-9/TIMP-1 ratio | 4 days | MMP-9/TIMP-1 is calculated from the measured plasma MMP-9 and TIMP-1 concentrations |
Countries
Hungary
Outcome results
None listed