Sickle Cell Disease
Conditions
Keywords
Sickle cell disease, Allo-immunization, Blood transfusion
Brief summary
Sickle cell patients have a high prevalence of alloimmunization. This high rate of alloimmunization can be partially explained by the existence of an antigenic difference between the predominantly Caucasian donor population and the sickle cell patients of African origin. Genetic and environmental risk factors have also been described. The main risk factors that have been shown in retrospective or cross-sectional studies are some HLA alleles, the age of the patient, the number of leukocyte-depleted erythrocyte concentrates (CED) transfused, the number of transfusion episodes, the age of the CEDs, the existence of an inflammatory event at the time of transfusion and the presence of anti-erythrocyte autoantibodies.There is also evidence of an impaired TH response but the underlying immunological mechanism is not fully understood. The aim of this study is to study the prevalence and the risk factors for anti-erythrocyte alloimmunization and to try to understand the immunological mechanisms.
Interventions
PROCEDUREBlood sampling
Extra blood sampling at the time of a blood transfusion in order to perform the laboratory analysis
Sponsors
Hanane EL KENZ
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
SCREENING
Masking
NONE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
Sickle cell disease patients treated within the CHU Brugmann or Queen Fabiola Children's Hospital
Exclusion criteria
None
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Lymphocyte typing | 1 hour before blood transfusion | Lymphocyte typing |
| Irregular antibodies | 1 hour before blood transfusion | Presence/abscence of irregular antibodies |
| C-reactive protein (CRP) | 1 hour before blood transfusion | CRP dosage |
| Cytokine | 1 hour before blood transfusion | Cytokine dosage |
| Heme oxygenase | 1 hour before blood transfusion | Heme oxygenase dosage |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Chronic or acute blood transfusion | 1 hour before blood transfusion | Blood transfusions planned at regular intervals of time (chronic transfusions) or performed in reaction to a medical issue (acute transfusion). |
| Blood transfusion indication | 1 hour before blood transfusion | Medical reason explaining the necessity of a blood transfusion |
| Blood donor ethnicity | 1 hour before blood transfusion | Blood donor ethnicity |
| Sex | 1 hour before blood transfusion | Sex |
Countries
Belgium
Outcome results
None listed