Microbiota, Ovarian Neoplasms, Ribosome Alteration
Conditions
Brief summary
This case-control study aims to compare the pelvic microbiomes of benign ovarian diseases and ovarian malignancies by 16s RNA techniques and culture. Discharges/flushing fluid from vagina, faces and fimbria end of fallopian tube are collected from age and menopausal status matched patient before and during procedures of laparoscopies. The discharges/flushing fluid would be sent for 16s RNA analysis and microculture respectively, and the results would get self-contrasted comparison and case-control comparison.
Interventions
DIAGNOSTIC_TESTmicrobiomes
16s RNA analysis and microculture of discharges/flushing fluid from vagina, faces and fimbria end of fallopian tube
Sponsors
Academy Military Medical Science, China
Lei Li
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Healthy volunteers
No
Inclusion criteria
* Laparoscopy procedures * Specific pathological diagnosis
Exclusion criteria
* Previous history of pelvic surgeries * Evidences of pelvic inflammation diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference of species distribution of microorganism according to 16s RNA analysis | 2 years | The difference of species of microorganism distribution according to 16s RNA sequencing analysis between control group and study group, and between different sampling sites in each arm |
| Difference of species distribution of microorganism according to microculture outcomes | 2 years | Difference of species distribution of microorganism according to microculture outcomes between control group and study group, and between different sampling sites in each arm |
Countries
China
Contacts
Primary ContactLei Li, MD
Backup ContactLan Zhu, MD
Outcome results
None listed