Adiposity, Inflammatory Response, Metabolism Disorder
Conditions
Keywords
elderly, exercise training, adipose tissue, omega-3 polyunsaturated fatty acids, inflammation, diferentiation of preadipocytes
Brief summary
The aim of this project is to investigate in elderly women, the effect of combined aerobic and resistive training and concomitant supplementation with omega-3 fatty acids on adipogenesis, metabolic functions and pro-inflammatory status of adipose tissue and on systemic metabolic profile.
Detailed description
Aging is associated with development of disturbances of lipid and carbohydrate metabolism and with systemic pro-inflammatory state similarly to the alterations observed in obese individuals. Metabolic and immune dysfunction of adipose tissue has been suggested as an important contributing trigger to this condition. EXODYA project is a clinical study in elderly individuals that aims to establish benefits of lifestyle intervention and nutritional supplementation -either alone or in combination- for the amelioration of systemic and adipose tissue metabolic and immune disturbances. Effects of 4 months´ intervention with combined aerobic plus resistive exercise training and supplementation with omega-3 polyunsaturated fatty acids on systemic metabolic profile and molecular characteristics of adipose tissue will be investigated in elderly women. Thus, a proof-of-concept will be provided for novel recommendations and guidelines for lifestyle interventions in elderly population
Interventions
BEHAVIORALExercise training
Combined aerobic and resistive training 3 times a week for 4 months. The strength training will be performed at moderate intensity (i.e., 50-60% of RM), and moderate volume (i.e., 2 to 3 sets per exercise). Endurance training will be performed at moderate- to high-intensity (i.e., 60-85% of VO2max) in the form of continuous and interval training (for high intensities), respectively. The training intensity will be prescribed individually on the basis of the initial exercise testing. Subjects will be thoroughly instructed on effective home-based exercise and advised to exercise in total 5 days a week.
DIETARY_SUPPLEMENTomega 3 supplementation
Capsules with high content of omega 3 fatty acids (Calanus oil) will be taken every day with lunch meal. The content of omega-3 fatty acids (EPA, DHA) per day should reach 250 mg.
DIETARY_SUPPLEMENTplacebo
Capsules with content of omega 6 fatty acids (sunflower oil) will be taken every day with lunch meal.
Sponsors
Charles University, Czech Republic
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Caregiver)
Masking description
Two types of pills (calanus or placebo) will be prescribed to subjects, investigator will randomly assign the subjects to the arms
Intervention model description
The exercise training intervention with or without omega-3 supplementation (Calanus oil) will be prescribed to non-trained sedentary elderly women
Eligibility
Sex/Gender
FEMALE
Age
60 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* elderly women: age 60-80 * non-trained (without regular physical activity) * BMI in the range of 25-30 kg/m2
Exclusion criteria
* diagnosed cancer, diabetes, liver and renal diseases, untreated hyper- or hypo-thyroidism, long term use of anti-inflammatory medication (anti-rheumatics and analgesics affecting cyclooxygenases (100 mg of anopyrin daily is acceptable), steroids and/or Omega-3 dietary supplements
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in adipose tissue inflammation | through study completion, an average of 1 year | analysis of distribution of immune cells (flow cytometry analysis) |
| Change in adipose tissue inflammatory signalling | through study completion, an average of 1 year | analysis of pro-resolving mediators (UPLC-MS/MS methodology) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in insulin sensitivity | through study completion, an average of 1 year | Hyperinsulinemic izoglycemic clamp (GDR-glucose disposal rate) |
| Change in physical performance | through study completion, an average of 1 year | Maximum graded exercise test on bicycle ergometer (Vo2max) |
| Change in gene expression in adipose tissue | through study completion, an average of 1 year | mRNA expression of metabolic and inflammatory genes in adipose tissue |
Countries
Czechia
Outcome results
None listed