Antigen-presenting Function, Sepsis, γδ T Cells
Conditions
Brief summary
Study on antigen-presenting function of gamma delta T cells in sepsis and its molecular mechanisms
Detailed description
The latest definition of sepsis highlights the role of dysregulated host response to infection in organ dysfunction aggravation. Maintenance of immune homeostasis of septic patients through immunotherapy is the key breakthrough to improve success rate. Antigen-presenting cell (APC) is the bridge that connects the innate and adaptive immunity. Decrease of count and function of APC has been shown to be associated with worsened clinical outcomes in patients with sepsis. Gamma-delta T cells (γδ T cells) are the newly identified population of T lymphocytes. Recent studies have found that γδ T cells possess unique and powerful antigen-presenting function, making them the hot topic in infection and cancer research. Based on the results, the investigator plan to evaluate the antigen-presenting function changes of γδ T cells in septic status by analyzing the antigen-presenting related molecules on γδ T cells, antigen protein uptake ability, and their function on the proliferation and activation of CD8+ T lymphocytes. Furthermore, the investigator will explore the mechanism and signal pathways for the APC function changes of γδ T cells. Findings from this research could help explain the mechanism of sepsis induced immune dysfunction, enrich our understanding of the important role of γδ T cells, and build a good foundation for immunotherapy in sepsis.
Interventions
OTHERCollect peripheral blood sample
Collect 20 ml heparinized blood from patients and controls strictly abiding by the principle of aseptic manipulation after informed consent.
Sponsors
West China Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
1. ≥18 year old; 2. a known or suspected infection based on clinical data at the time of admission and sepsis-induced dysfunction of at least one organ.
Exclusion criteria
1. autoimmune disease, 2. history of transplantation, 3. acute tuberculosis, 4. chronic hepatitis B or C infection, 5. HIV infection, 6. active malignancy, and 7. long-term use of cortical steroids.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 28-day clinical outcome | 28-day after ICU admission | death |
Contacts
Primary ContactXuelian Liao, MD
Outcome results
None listed