Lower Resp Tract Infection, Microbiota, Pneumonia, Bacterial, Respiratory Failure
Conditions
Keywords
Microbiota, Respiratory Failure, Pneumonia, Lower Respiratory Tract Infection
Brief summary
A prospective observational study. Enrolled participants admitted to ICU due to pneumonia and respiratory failure need mechanical ventilator support. Investigators collected the residual specimens, such as sputum from endotrachea aspiration, bronchoalveolar lavage fluid in those participants as the usual care in the ICU. Those residual samples were sent to extract RNA and sequence by using high-throughput sequencing (next-generation sequencing) method. Investigators will compared the microbiome feature between lower respiratory tract and stool specimens in those participants diagnosed as pneumonia with respiratory failure.
Interventions
DIAGNOSTIC_TEST16s RNA sequence of sputum, bronchoalveolar lavage fluid (options), stool
We will check the microbiome in lower respiratory samples (sputum and bronchoalveolar lavage fluid) and stool by using 16S RNA sequencing
Sponsors
Taichung Veterans General Hospital
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
40 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Adults aged than 40 years old * Diagnosed as pneumonia * Respiratory failure with mechanical ventilator support
Exclusion criteria
* Less than 40 years old * Has been treated with antibiotics more than 7 days within two weeks before enrolling into study * Suspected or active pulmonary tuberculosis infection
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The dynamics of the airway bacterial microbiome in the lower respiratory tract specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation) | Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7 | Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S rRNA sequence of bacteria in residual samples |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The dynamics of the gut bacterial microbiome in the stool specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation) | Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7 | uses high-throughput sequencing (next-generation sequencing) to sequence the 16S |
| The association of the microbiome between lower respiratory tracts and stool specimens in pneumonic patient with respiratory failure | Compared the association of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7 | Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S |
Countries
Taiwan
Outcome results
None listed