Microcirculation
Conditions
Brief summary
The objective of the current study is to investigate the hemodynamic changes in the peripheral microcirculation system as a response to various changes, using noninvasive thermography and laser doppler
Detailed description
This study includes a single session per subject. Microvascular and systemic variables will be monitored in response to a number of non-invasive interventions/device that are approved for clinical use and known to elicit microvascular responses. These interventions are applied consecutively, include breathing at different rates, low power visible light in the red-to-near-infrared range, increase in local temperature, and changes in the arm position.
Interventions
DEVICEOmnilux new-U (Red LED)
The left wrist (including area of radial artery) will be illuminated with the Omnilux new-U (Red LED) light source, while the right wrist will not be illuminated
The left wrist (including area of radial artery) will be illuminated with the Omnilux new-U (Near infrared LED) light source, while the right wrist will not be illuminated
DEVICERESPeRATE
The subjects will be guided by a digital metronome to breath at 15, 10, and 6 breaths/min for 2 minutes each using the metronome function of the device RESPeRATE (InterCure, Israel) that guides breathing using musical tones played at selectable rhythm
DEVICEHeating bag
Applying a standard 10X13 \[cm\] heating bag on the surface of the hand at 43-45 degree Celsius for 10 seconds
Sponsors
Hebrew University of Jerusalem
Afeka, The Tel-Aviv Academic College of Engineering
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
23 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
1. Healthy males and females, between 23 and 45 years of age. 2. Willing to sign informed consent
Exclusion criteria
1. Currently smoking 2. Any abnormal skin condition in the area of light irradiation. 3. Pregnant having given birth less than 3 months ago, and/or breastfeeding. 4. Having a history of diseases stimulated by heat, such as recurrent Herpes Simplex in the treated area, unless treatment is conducted following a prophylactic regimen. 5. Having any illness that might affect the vasculature, such as diabetes (type I or II) 6. Suffering from significant concurrent illness, such as cardiac disorders or pertinent neurological disorders. 7. As per the Investigator's discretion, any physical or mental condition which might make it unsafe for the subject to participate in this study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Capillary blood flow | Up to 1 hour | Capillary blood flow (red blood cell velocity or flux in 'perfusion units') as measured by laser doppler (Biopac™ System, Inc, USA). This is a continuous measurement (at least 10 Hz sampling rate) from baseline to end of experiment. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Temperature distribution over hands measured by thermography | Up to 1 hour | Thermographic images will be photographed with a FLIR camera and temperature distribution will be analysed. Photos will be taken every minute from baseline to end of experiment. |
| Tissue oxygenation (tcPO2) | Up to 1 hour | Tissue oxygenation (tcPO2) measured by electrodes mounted on both hands (Perimed™ system, Sweden). This is a continuous measurement (at least 10 Hz sampling rate) from baseline to end of experiment. |
Countries
Israel
Outcome results
None listed