Clinical Pregnancy Rate
Conditions
Keywords
embryo transfer, volume, culture medium, loading the catheter, clinical pregnancy rate
Brief summary
The aim of this study was to evaluate if higher volume (40-45μl VS 20-25 μl) of media used for embryo transfer affects the clinical outcomes in fresh IVF cycles.Patients who fulfilled the inclusion criteria and agreed to participate in the study, were randomized, on the day of the embryo transfer, into group A: low volume (20-25 μL) and group B: high volume (40-45 μL)
Detailed description
The volume of culture medium used for embryo transfer is a variable that has been speculated to affect the IVF outcome. It is known that ultra high volume (\>100 μl) of medium during embryo transfer may result in embryo expulsion out of the uterus or ectopic pregnancy. Similarly, some authors advocate that ultra low volume of transferring medium (\<10 μl) may negatively affect pregnancy and implantation rates. However small differences in the volume of the culture medium may favor the outcome of the embryo transfer. The aim of this study was to evaluate if the use higher volume (40-45μl VS 20-25 μl) of media used for embryo transfer affects the clinical outcomes in fresh IVF cycles.
Interventions
OTHERVolume of medium during embryo transfer
Sponsors
Aretaieion University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
1. Age ≤ 45y.o. 2. BMI≤36 kg/m2, 3. Baseline FSH concentration \<15 IU/l, 4. Ovarian stimulation with the same GnRH antagonist protocol 5. Normal uterine cavity with endometrium thickness \>7mm and trilaminar morphology at the Time of transfer and semen 6. Parameters of \>1 million /ml motile spermatozoa with \>4% physiological morphology -
Exclusion criteria
1. PGD cycles 2. Frozen -thaw cycles 3. Natural cycles or patients following different ovarian stimulation protocol were excluded from the study \-
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clinical pregnancy rate | 25 days after embryo transfer | Number of clinical pregnancies expressed per 100 embryo transfer cycles |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Implantation rate | 25 days after embryo transfer | The number of gestational sacs observed divided by the number of embryos transferred. |
| Ongoing pregnancy rate | 20 weeks | Number of pregnancies till 20 weeks of gestation expressed per 100 embryo transfer cycles |
Outcome results
None listed