Diabetes Mellitus, Type 2, Hypertension, Vitamin D Deficiency
Conditions
Brief summary
This trial will evaluate whether a particular type of circulating white blood cell, monocytes, from type 2 diabetics with high blood pressure and vitamin D deficiency vs. sufficiency will induce hormones that increase blood pressure.
Detailed description
More than half of patients with type 2 diabetes mellitus develop hypertension, which doubles their risk for cardiovascular disease. Inflammation plays a role in the development of these diseases, and monocytes, a type of white blood cell, may be critical. This study will isolate monocytes from blood samples of patients with type 2 diabetes and high blood pressure who have either high levels of vitamin D or low levels of vitamin D, and determine what their effects are on stimulating production in kidney cells of a hormone that increases blood pressure.
Interventions
DIAGNOSTIC_TESTBlood draw
Blood will be drawn to isolate monocytes after 2 weeks off of blood pressure lowering medications.
Sponsors
Washington University School of Medicine
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
25 Years to 80 Years
Inclusion criteria
* Type 2 diabetes (T2DM) * Age: 25-80 years old * Vitamin 25(OH)D3 levels \<20 or \>30 ng/ml. * HbA1c: 5.5%-9.5% * Blood Pressure: \<160/100 mm Hg on BP lowering medications and * Blood Pressure: \>120/80 to 160/100 mmHg without BP lowering medications
Exclusion criteria
* Recent weight loss of more than 5% within 3 months prior to screening * Current Pregnancy * Hypercalcemia * Previous heart attack and stroke * Heart failure * Atrial fibrillation * Heavy alcohol consumption * Extreme diets (Atkins, South Beach) * Chronic renal failure: stage 4 or worse * \>2 + proteinuria on urine sample * Chronic diseases known to affect immunity
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Renin production by monocytes | 2 weeks | Blood will be collected for isolation of monocytes. These cells will then be cultured to determine whether there is monocyte stimulation of a hormone that induces high blood pressure (renin) in kidney cells. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Renin production by monocyte media | 2 weeks | Blood will be collected for isolation of monocytes. The media from these cells will then be added to culture to determine whether there is stimulation of a hormone that induces high blood pressure (renin) in kidney cells. |
Countries
United States
Outcome results
None listed