HIV/AIDS
Conditions
Keywords
Vascular function, Physical training, Laser Speckle Contrast Imaging
Brief summary
Introduction: It is well known that the infection caused by the Human Immunodeficiency Virus (HIV) is associated to a higher cardiovascular event risk. On the other hand, it is clear that the aerobic exercise training induces improvements in autonomic control and vascular function, through increases in vasodilator agents and blood vessels number. However, the investigators could not find previous works that studied the microvascular function in response to exercise training in HIV patients. Objectives: To study the impact of aerobic exercise training in endothelial function of HIV patients. In addition, the investigators will verify the association of the physical fitness to the nitric oxide bioavailability, angiogenesis and lipid profile in HIV patients. Methods: The study subjects will be composed by HIV patients, that will be randomly divided in two different groups: exercised and sedentary. The subjects will be tested before and after training in regards to endothelial function, nitric oxide bioavailability, physical fitness and lipid profile, through flowmetry, colorimetric essays, maximal cardiopulmonary exercise test, and biochemical tests; respectively. The exercise training will be performed in a treadmill for 12 weeks, 3 times a week, 40 minutes each section. Expected Results: The investigators expect to achieve markers that will help in understanding the interaction of HIV with several factors that contribute to an increased endothelial function after exercise training.
Detailed description
HIV-infection triggers a massive oxygen reactive species production, increasing oxidative stress and vascular complications. Despite the importance of therapeutic strategies in reducing the cardiovascular risk among HIV-infected patients (HIVP), exercise-related effects upon macro-and-microvascular endothelial function and oxidative stress in HIVP have not been previously reported.
Interventions
BEHAVIORALExercise
A multimodal exercise training program (aerobic, strength and flexibility exercises) for 6 months (60-min sessions performed 3 times/week with moderate intensity).
Sponsors
Rio de Janeiro State University
Instituto Nacional de Cardiologia de Laranjeiras
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* sedentarism for at least 6 months; * diagnosis of HIV infection.
Exclusion criteria
* opportunistic diseases; * nephropathies; * cardiovascular diseases; * locomotor limitations; * liver diseases.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Skin microvascular blood flow | Baseline and 3 months | Microvascular reactivity will be evaluated by laser speckle contrast imaging with laser wavelength of 785 nm in combination with iontophoresis of acetylcholine (ACh) and sodium nitroprusside (SNP) for noninvasive and continuous measurements of cutaneous microvascular flow changes in the forearm. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Peak Oxygen consumption | Baseline and 3 months | Measured at a maximal graded exercise test. |
| Nitrite/nitrate | Baseline and 3 months | Colorimetric assay kit |
| Body fat | Baseline and 3 months | Dual-energy X-ray absorptiometry. |
| Forearm macrovascular blood flow | Baseline and 3 months | Forearm macrovascular blood flow will be evaluated by venous occlusion plethysmography. |
| Capillary density | Baseline and 3 months | Capillary density will be evaluated by high-resolution intra-vital color microscopy. |
Countries
Brazil
Outcome results
None listed