Periodontal Diseases, Pregnancy Gingivitis
Conditions
Keywords
chromogranin A, stress, gingivitis, pregnancy
Brief summary
Background: The purpose of the present study is to research whether stress scale scores alter with relationship to gingival inflammation and examine the impact of non-surgical periodontal therapy during pregnancy on the levels of cytokines in gingival crevicular fluid (GCF) and on salivary stress-related hormones. Methods: 30 non-pregnant (control group) and 30 pregnant women (test group) who fulfilled the study inclusion criteria were chosen. The participants with moderate/severe gingivitis were included. Clinical data and samples of GCF and salivary were collected at baseline and after periodontal therapy. The levels of cytokines interleukin-1 beta (IL-1β) and IL-10, and concentration of salivary chromogranin A (Cg A) hormone were analyzed by enzyme-linked immunosorbent assay kits.
Detailed description
Periodontal clinical measurements and periodontal treatment were applied by a previously trained and calibrated examiner. Clinical parameters measurements including; probing pocket depths (PPD; the distance from the gingival margin to the base of the gingival sulcus), clinical attachment level (CAL), full mouth gingival index (GI)21 and plaque index (PI)20 scores using a periodontal probe were registered following saliva and GCF sample collection. Then, non-surgical periodontal therapy (NPT) consisted of scaling, root planning, and oral hygiene instruction was applied. Clinical measurements, recruitment of GCF, and saliva samples were repeated after three weeks. Gingival Crevicular Fluid and Saliva Sample Collection Selected anterior teeth for GCF sampling were isolated from saliva using cotton rolls, a gentle stream of air was conducted parallel to the root surface for 5 to 10 seconds to dry the area. The periopaper strips were gently inserted into the gingival crevice until resistance was felt and were left in place for 30 s and were collected from mesio-buccal sulcus of teeth in the anterior region of each patient (two samples per patient and per visit). The samples containing blood were discarded and were placed in sterile Eppendorf tubes and were stored at -20°C until the assay procedure.
Interventions
PROCEDUREperiodontal treatment
non-surgical periodontal therapy (NPT) consisted of scaling, root planning, and oral hygiene instruction
Sponsors
Tokat Gaziosmanpasa University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
1. Must be systemically healthy; 2. Must be at least twenty teeth in the mouth; 3. Clinical diagnosis of gingivitis; 4. Must be the probing pocket depth (PPD)≤3 mm in all four quadrants.
Exclusion criteria
* the use of anti-inflammatory, * antimicrobial and hormone therapy within the preceding 6 months, * having psychiatric disorders, * a systemic disease, * smoking cigarettes and * for the control group breastfeeding, pregnancy and menstrual periods.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| salivary Cg A hormone concentration | up to 8 weeks | Samples should be taken before and after treatment and evaluated using ELISA kits |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| IL-1β and IL-10 Cytokine levels in the GCF | up to 8 weeks | Samples should be taken before and after treatment and evaluated using ELISA kits |
Outcome results
None listed