Physical Fitness as Klotho Protein Stimulator.

Conditions

Aging, Physical Activity

Keywords

Physical fitness, Klotho, Training, HIIT, WB-EMS

Brief summary

FIT-AGING will determine the effect of different exercise modalities on α-Klotho protein (primary outcome) in sedentary healthy adults. FIT-AGING also investigate the physiological consequences of activating Klotho gene (secondary outcomes).

Detailed description

Aims: The plasma Klotho protein concentration (α-Klotho protein), which is considered a powerful biomarker of longevity, makes it an attractive target as an anti-aging therapy against functional decline, sarcopenic obesity, metabolic and cardiovascular diseases, osteoporosis, and neurodegenerative disorders. Physical exercise and physical fitness, could be α-Klotho protein activators through biochemical process. FIT-AGING will determine the effect of different exercise modalities on α-Klotho protein (primary outcome) in sedentary healthy adults. FIT-AGING also investigate the physiological consequences of activating Klotho gene (secondary outcomes). Methods: FIT-AGING will recruit 60 sedentary, healthy, adults (50% women) aged 40-65 years. Eligible participants will be randomly assigned to a non-exercise group to the usual control group (n=15), physical activity recommendation from American College of Sport Medicine, World Health Organization and American Heart Association group (n=15), High Intensity Interval Training group (n=15)) and Whole-Body Electromyostimulation group (n=15). Laboratory measures completed at baseline and 12 weeks later, include α-Klotho protein concentration, physical fitness (cardiorespiratory fitness, muscular strength), body composition, resting metabolic rate, hearth rate variability (HRV), health blood biomarkers, free-living physical activity, sleep habits reaction time, cognitive variables and health-related questionnaires. The investigators will also obtain dietary habits data and cardiovascular disease risk factors.

Navarro-Lomas G, Dote-Montero M, Alcantara JMA, Plaza-Florido A, Castillo MJ, Amaro-Gahete FJ.

2022-05-1013 citations

10.1007/s00421-022-04957-9

The effects of three types of exercise training on steroid hormones in physically inactive middle-aged adults: a randomized controlled trial.

Dote-Montero M, De-la-O A, Jurado-Fasoli L, Ruiz JR, Castillo MJ, Amaro-Gahete FJ.

2021-04-229 citations

10.1007/s00421-021-04692-7

1,25-Dihydroxyvitamin D and S-Klotho Plasma Levels: The Relationship Between Two Renal Antiaging Biomarkers Mediated by Bone Mineral Density in Middle-Aged Sedentary Adults

Alejandro De‐la‐O, Lucas Jurado‐Fasoli, Manuel J. Castillo, Ángel Gutiérrez, Francisco J. Amaro‐Gahete

Rejuvenation Research2021-01-257 citations

10.1089/rej.2020.2384

Relationships between diet and basal fat oxidation and maximal fat oxidation during exercise in sedentary adults.

Jurado-Fasoli L, Amaro-Gahete FJ, Merchan-Ramirez E, Labayen I, Ruiz JR.

2020-12-0112 citations

10.1016/j.numecd.2020.11.021

Interventions

OTHERPAR

OTHERHIIT

OTHERWB-EMS

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

PREVENTION

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

40 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

* Age: 40-65 years * BMI:18.5-35 kg/m2 * Not engaged in regular physical activity \>20min on \>3days/week * Not participating in a weight loss programme * Stable weight over the last 5 months (body weight changes\>5kg) * Participant must be capable and willing to provide consent, understand

Exclusion criteria

and accept the randomized group assignment * Normal electrocardiogram

Design outcomes

Primary

Measure	Time frame	Description
α-Klotho protein	Baseline and through study completion, an average of 12 weeks.	Plasma Klotho concentrations will be measured by ELISA using a soluble α-Klotho ELISA assay kit.

Secondary

Measure	Time frame	Description
Physical fitness: muscular strength.	Baseline and post-intervention.Baseline and through study completion, an average of 12 weeks.	Muscle strength measure through: Isokinetic strength, Handgrip strength and Core resistance stability.
Body composition.	Baseline and through study completion, an average of 12 weeks.	Measure by DXA.
Resting energy expenditure.	Baseline and through study completion, an average of 12 weeks.	Evaluated by indirect calorimetry with gas analyzer breath by breath.
Hearth Rate Variability (HRV).	Baseline and through study completion, an average of 12 weeks.	Measure by Polar RS800CX.
Biochemical profile.	Baseline and through study completion, an average of 12 weeks.	General biochemical profile in blood samples
Physical activity.	Baseline and through study completion, an average of 12 weeks.	The amount of physical activity will be measured by accelerometer.
Physical fitness: cardiorespiratory fitness	Baseline and through study completion, an average of 12 weeks.	Cardiorespiratory fitness measure through a maximum treadmill test (VO2max.).
Cognitive test.	Baseline and through study completion, an average of 12 weeks.	Auditory memory tests
Dietary assessment.	Baseline and post-Baseline and through study completion, an average of 12 weeks..	Measure by 24h recall and Food Frecuency Questionnaire.
Anthopometric measures.	Baseline and post-Baseline and through study completion, an average of 12 weeks..	Measure by stadiometer and scale.
Sleep quality.	Baseline and post-Baseline and through study completion, an average of 12 weeks..	The quality of sleep will be measured by accelerometer.
Hormones profile	Baseline and post-Baseline and through study completion, an average of 12 weeks..	Hormones profile measure in blood samples.
Reaction time.	Baseline and through study completion, an average of 12 weeks.	Measure by Vienna System.

Outcome results

None listed