Field Cancerization for Cannabis Smokers
Conditions
Brief summary
The aim of this study is to detect P53 expression in clinically normal oral mucosa of cigarette smokers, simultaneous cigarette and cannabis smokers and non-smokers as a surrogate marker for field cancerization.
Detailed description
Roles and Responsibilities: 1. Ali Abd El Wahed Abd El Fatah Kotb (A.K.) operator, data enterer, Assistant lecturer, Oral and Maxillofacial Pathology, Faculty of Oral and Dental Medicine, Cairo university, Egypt. 2. Professor Dr. Heba Dahmoush (H.D.) main supervisor, data monitoring,auditing professor, Oral and Maxillofacial Pathology, Faculty of Oral and Dental Medicine, Cairo university, Egypt. 3. Eman Desoky (E.D.) sample size calculation, statistician,Faculty of Oral and Dental Medicine, Cairo University, Egypt. 4. Evidence Based Dentistry Committee (CEBD)help in reporting study protocol following SPIRIT guidelines,Faculty of Oral and Dental Medicine, Cairo University, Egypt. 5. Research Ethics Comittee (CREC)protocol reviewer in order to protect the right, safety, dignity and well-being of the participants, Faculty of Oral and Dental Medicine, Cairo University, Egypt. 1- Intervention: A.K. will collect specimens from cigarette smokers and simultaneous cigarette and cannabis smokers. then the expression of p53 as a marker for field cancerization will be investigated in formaline fixed paraffine embedded specimens of mucosal biopsies obtained from the oral mucosa of both groups. 2-comparator: mucosal biopsies from non smokers will be investigated as a copmaratitive to smokers group.
Interventions
OTHERCigarette smokers
frequent cigarette smoking for not less than 5 years
OTHERSimultaneous cigarette and cannabis smokers
frequent cigarette and cannabis smoking for not less than 5 years
Sponsors
Cairo University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* frequent smokers (at least 10 cigarettes a day) for a period not less than 5 years. * Simultaneous smokers to be included in the study must be frequent cigarette smokers (at least 10 cigarettes a day) for a period not less than 5 years plus frequent cannabis smoker (at least 3 times per week) for a period not less than 5 years.
Exclusion criteria
* Exposure to other carcinogens. * un-clear smoking history. * medically compromised patients
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| P53 expression as a surrogate marker for field cancerization | not less than 5 years | The areas containing the most uniformly stained tissues will be chosen for evaluation in area-area/area-percent measurments |
Outcome results
None listed