Impaired Mitochondrial Function, Muscle Performance
Conditions
Brief summary
As people grow older skeletal muscle gradually becomes smaller and weaker, causing reduced mobility and quality of life. To understand and reverse this negative process investigators need to find new ways of improving the ability of muscle to perform physical activity. There is some evidence that supplements may improve how the mitochondria work, and investigators want to explore this idea in more detail. This is possible by measuring how the muscles work and respond to exercise before and after taking the supplement alongside an aerobic (i.e. cycling) and resistance (i.e. weight lifting) exercise programme. This will give us the basic information investigators would need to see if this is a useful idea.
Interventions
DIETARY_SUPPLEMENTAcipimox
Acipimox plus exercise training: Oral supplement containing Acipimox 250mgs as the active ingredient in blinded label tablet form.
DIETARY_SUPPLEMENTPlacebo
Alternate unilateral resistance and aerobic exercise training will also be performed 5 times per week for 6 weeks.
Sponsors
Royal Devon and Exeter NHS Foundation Trust
University of Exeter
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
65 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Male * Age between 65-75 years * Body mass index between 19-29 * No active cardiovascular or metabolic disease * No active respiratory disease * No current musculoskeletal injuries * A sedentary lifestyle (i.e. does not engage in strenuous, planned physical activity) * The ability to give informed consent
Exclusion criteria
* Currently taking a statin drug or NSAIDs * Have a current peptic ulcer * Have any renal impairment * Have a known hypersensitivity to Acipimox * Suffer from vertigo * Smoker
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in mitochondrial function | Baseline, 3 weeks, 6 weeks | Mitochondria will be extracted from muscle samples immediately post-biopsy (biopsies taken baseline, week 3 and week 6) and analysed for content and subsequently for oxidative respiratory function using the Oroboros technique, and maximal rates of Adenosine Triphosphate (ATP) production. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Chronic changes in habitual muscle protein synthetic rates | Baseline, 3 weeks and 6 weeks | Baseline saliva samples then frequent saliva samples over 6 weeks following oral ingestion of the stable isotope deuterium oxide (D2O, or 'heavy water') will be analysed by gas-chromatography-pyrolysis-isotope ratio mass-spectrometry. analysis. Muscle samples collected at baseline, 3 weeks, 6 weeks will also be analysed by gas-chromatography-pyrolysis-isotope ratio mass-spectrometry |
Countries
United Kingdom
Outcome results
None listed