Embryo Development
Conditions
Keywords
preimplantation development, DNA fragmentation, abstinence duration
Brief summary
The aim the investigators study is to analyze the embryo development if oocytes of the participants are injected with sperm from a longer or shorter (2 hours) abstinence duration.
Detailed description
Participants undergoing an IVF treatment, are asked to respect an abstinence duration of 2-7 days. This is to obtain a sufficient number of motile sperm. A recent investigation, analyzed the quality (number and motility) of a sperm sample produced after an abstinence duration of 4 to 7 days, followed by a second sperm sample 2 hours later. The second sample (after 2 hours) was able to produce sperm cells with a higher motility. Not only has this short abstinence duration of 2 hours a positive effect on the motility, it will also reduce the sperm DNA damage, which again has a positive effect on embryo development. Because participants need gametes of high quality to obtain embryos with a high quality, the investigators want to investigate what the effect is of abstinence duration on embryo development.
Interventions
OTHERsecond masturbation
Husbands will be asked to produce a second sperm sample in the masturbatorium
Sponsors
Universitair Ziekenhuis Brussel
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Intervention model description
a prospective sibling oocyte study: both study arms will be applied to the oocytes of 1 patient
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Patients planned for embryo development up to day 5 * at least 6 mature oocytes * fresh ejaculates * ICSI * fresh embryo transfer or freeze all strategy * all stimulation protocols * concentration of \>1x10\^6 /ml sperm
Exclusion criteria
* IVF * IVF versus ICSI patients * chirurgical sperm * acceptors of donor oocytes * patients with PGD * patients already enrolled in another study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Embryo development (blastocyst stage) | 1 year | embryo development 5/6 of the preimplantation development |
| Embryo development (cleavage stage) | 1 year | embryo development on day 3 of the preimplantation development |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Sperm quality | 1 year | concentration and motility |
| Fertilization | 1 year | number of fertilized oocytes per number of mature oocytes |
| Clinical Pregnancies | 1 year | pregnancies with a fetal heart beat |
| Sperm DNA fragmentation | 1 year | analysis of non-viable sperm in the two samples |
| Utilization rate | 1 year | number of embryos used for transfer + cryopreserved for the patient |
Countries
Belgium
Outcome results
None listed