Healthy
Conditions
Keywords
exercise, recovery, muscle, protein
Brief summary
The primary objective is to determine the impact of essential amino acids on integrated muscle protein synthesis over 4 days after resistance exercise. In addition, the secondary objective is to determine the effect of essential amino acids on satellite cell regulation and inflammatory responses during this prolonged recovery period.
Interventions
DIETARY_SUPPLEMENTActive
4g of essential amino acids
DIETARY_SUPPLEMENTPlacebo
4g of maltdextrin
Sponsors
Ajinomoto Co., Inc.
University of Toronto
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy, male, recreationally-active participants * Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix. B). Active will be defined as being in the top 20% of age specific scores on iPAQ (the International Physical Activity Questionnaire; Appendix.C). * Participants are required not to engage in lower limb RT (resistance training) for at least 6 months prior to the study. * Participants will be 18-35 years old. * Participants are willing to abide by the compliance rules of this study
Exclusion criteria
* Female * Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor * Self-reported regular tobacco use * Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.) * The individual who has allergy for milk
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle protein synthesis | 4 hours | Skeletal muscle fractional synthesis rate (%/h) will be determined by oral pulse method using ring-\[D5\]phenylalanine |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| exercise performance | 4 days | Maximal strength (torque in n-M) of the knee extensors will be assessed by a maximal isometric voluntary contraction (MVC). |
| Muscle soreness | 4 days | Corresponding subjective ratings of muscle soreness will be collected from participants using a visual analogue scale (mm) |
| Muscle damage | 4 days | Muscle damage will be determined by histochemical analysis. muscle damage is defined as Z-band streaming. |
Countries
Canada
Outcome results
None listed