Healthy
Conditions
Brief summary
This study investigates the relation between ion-handling (K+) and fatigue development during exercise. Before and after a 6-week period of high intensity interval training, performance and ion-handling is assessed in young adults. In addition, mitochondrial function is examined.
Interventions
BEHAVIORALSpeed endurance training
Speed endurance training
Sponsors
Morten Hostrup, PhD
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men * 45-55 ml/kg/min * BMI 19-26 kg/m2 * Normal ECG (assessed by MD)
Exclusion criteria
* Allergic to n-acetylcystine (NAC) or digoxin * Severe side effects from NAC or digoxin * Chronic diseases (assessed by MD to be a potential hazard or to interfere with outcome measures)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Quadriceps endurance | Change from before (baseline) to after 6 weeks of training (Week 6) | Time to exhaustion during knee-extensor exercise |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| K+ release | Change from before (baseline) to after 6 weeks of training (Week 6) | Net leg K+ release in mmol/min during knee-extensor exercise |
Other
| Measure | Time frame | Description |
|---|---|---|
| Muscle Na+/K+-ATPase subunit content | Change from before (baseline) to after 6 weeks of training (Week 6) | Muscle Na+/K+-ATPase subunit content from vastus lateralis biopsies |
| Femoral venous plasma K+ | Change from before (baseline) to after 6 weeks of training (Week 6) | Femoral venous plasma K+ in mmol/L during knee-extensor exercise |
| Muscle mitochondrial content | Change from before (baseline) to after 6 weeks of training (Week 6) | Muscle mitochondrial content of citrate synthase from vastus lateralis biopsies |
| Muscle mitochondrial respiration | Change from before (baseline) to after 6 weeks of training (Week 6) | Muscle mitochondrial respiration by high resolution respirometry (flux per mg muscle) from vastus lateralis biopsies |
| Femoral venous lactate | Change from before (baseline) to after 6 weeks of training (Week 6) | Femoral venous lactate in mmol/L during knee-extensor exercise |
Countries
Denmark
Outcome results
None listed