Cardiac Surgery
Conditions
Keywords
HLA-DR expression, cardiopulmonary bypass, neonate
Brief summary
The purpose of this study is to determine how monocyte HLA-DR and other markers of immune function change with time in neonates who underwent cardiac surgery with cardiopulmonary bypasss. We hypothesize that HLA-DR expression in neonates is predictive of septic complications after cardiopulmonary bypass
Interventions
OTHERBlood collection
A sample of an additional blood tube will be taken in parallel with the blood test carried out for the treatment.
Sponsors
Fédération Française de Cardiologie
Nantes University Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 28 Days
Healthy volunteers
No
Inclusion criteria
* Patients aged ≤ 28 days * Admission after cardiac surgery under cardiopulmonary bypass
Exclusion criteria
* Lack of parental consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| HLA-DR expression | Preoperatively, and 1, 2, 3 and 4 days after the end of cardiopulmonary bypass | Number of HLA-DR molecules per monocyte (AB/c) determined by flow cytometry on whole blood using a Quantibrite phycoerythrin fluorescence quantitation kit (BD Biosciences) Number of HLA-DR molecules per monocyte (AB/c) determined by flow cytometry on whole blood using a Quantibrite phycoerythrin fluorescence quantitation kit (BD Biosciences) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cytokines measurements | Preoperatively, and 1, 2, 3 and 4 days after the end of cardiopulmonary bypass | Pro and anti-inflammatory cytokine concentrations in the plasma measured by multiplex immunoassay |
Countries
France
Outcome results
None listed