House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

Effects and Mechanism of House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

Status

Withdrawn

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03307278

Enrollment

Registered

2017-10-11

Start date

2017-11-30

Completion date

2018-09-06

Last updated

2021-08-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Allergic Asthma

Brief summary

In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.

Interventions

BIOLOGICALHouse dust mite

As a stimulant to induce inflammasome activation

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

20 Years to 70 Years

Healthy volunteers

Yes

Inclusion criteria

* HDM allergic and non allergic subjects

Exclusion criteria

* Pregnant subject, cancer

Design outcomes

Primary

Measure	Time frame	Description
inflammasome activation by HDM	6 hours	The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
Steroid receptor phosphorylation by HDM	6 hours	The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026