Peripheral Artery Disease
Conditions
Brief summary
The measurement of the transcutaneous oxygen pressure during walking allows to estimate the importance of ischemia, bilaterally and segment of limb by segment of limb. The determination of the metabolic compounds concentration, with metabolomic approach, is emerging in physiology and exercise. This pilot study focuses on the feasibility of the metabolomics analysis with micro method , by capillary drawing from earlobe sampling, for patients with peripheral artery disease (PAD)
Detailed description
Ankle brachial index is measured at rest for alml subjects. Blood sample (180-360 microliter) will be collected at rest transcutaneous oxygen pressure is measured at the chest, calves and buttocks and results will be expressed as the minimal value of the DROP index (Limb changes minus chest changes) Patients walk on treadmill (2MPH 10% slope) until exertional limb pain. Blood sample (180 microliter) will be collected again, at minute 3 of recovery from walking Correlation of each metobolite increase to the sum of DROP values wil be studied.
Interventions
BIOLOGICALCapillary blood sampling from the earlobe
Sample at rest before the walking test Sample at rest 3 minutes after the end of the walking period
Sponsors
University Hospital, Angers
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Masking description
Metabolomic analysis will be performed blinded to the exercise test results
Intervention model description
Analysis of changes in metabolomic signature from rest to post exercise
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Age \> 18 years old * Patient able to walk on a treadmill * Patient agrees to be involve in the protocol and sign the consent form
Exclusion criteria
* Patient not covered by the French health insurance * Patient has a wash-out period for another clinical trial * Patient with comorbidity as bleeding disorder or with a anticoagulant therapy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Chances in the metabolomic signature of arterial claudication by capillary blood sampling from the earlobe | Half an hour (includes the resting walking and recovery period of exercise) | Search a significant difference between biological markers before and after the test during a walk on a treadmill |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes inthe correlation of observed metabolomic variations and the importance of ischemia evaluated by oximetry | Half an hour (includes the resting walking and recovery period of exercise) | Evaluate the coefficient of correlation between the changes of metabolomic pârameters and the sum of the observed DROPmin values of all studied subjects. |
Countries
France
Outcome results
None listed