Amino Acid, Muscle Protein Synthesis
Conditions
Keywords
Carbohydrate, Cluster Dextrin, Exercise training, Protein supplement
Brief summary
10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin. The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin. Hypotheses: I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period. II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.
Interventions
DIETARY_SUPPLEMENTMeat Hydrolysate
25 grams of meat hydrolysate to maximally stimulate protein synthesis.
DIETARY_SUPPLEMENTCluster Dextrin
75 grams of Cluster Dextrin
DIETARY_SUPPLEMENTPlacebo
75 grams of Glucose
Sponsors
University of Birmingham
Bispebjerg Hospital
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* young healthy adults, * organised sport/training 1-3 times per week
Exclusion criteria
* Subject younger than 18 years or above 30 years * BMI \> 30 * Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus. * Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week. * Participating in systematized exercise more than 3 times per week. * Vegetarian. * Participating in a special diet which deviates from the standard of young adults.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Protein hydrolysate amino acid label time to peak appearance in plasma | From hour 0 to hour 3 | Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise | from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3. | Difference between myofibrillar protein fractional synthesis rate after meat hydrolysate with either 75 grams of Cluster Dextrin or Clucose. |
| Protein hydrolysate amino acid label area under the concentration curve in plasma | From hour 0 to hour 3 | Difference between the area under the concentration curve of protein-derived D5-phenylalanine amino acid appearance in the plasma |
| Insulin concentration | From hour 0 to hour 3 | Difference between insulin concentrations after intake of the two carbohydrate types. |
Countries
Denmark
Outcome results
None listed