Colorectal Cancer, Hyperplastic Polyp, Serrated Polyp, Sessile Serrated Adenoma
Conditions
Brief summary
Gut microbiota were assessed in 540 colonoscopy-screened adults by 16S rRNA gene sequencing of stool samples. Investigators compared gut microbiota diversity, overall composition, and normalized taxon abundance among these groups.
Detailed description
Colorectal cancer is a heterogeneous disease arising from at least two precursors-the conventional adenoma (CA) and the serrated polyp. A relationship between the human gut microbiota and colorectal cancer has been shown; however, its relationship to the different early precursors of colorectal cancer is understudied. This study tested, for the first time, the relationship of the gut microbiota to specific colorectal polyp types.
Interventions
DIAGNOSTIC_TESTMobio PowerSoil DNA Isolation Kit
16S rRNA amplicons covering variable regions V3 to V4 were generated using primers (347F-5'GGAGGCAGCAGTRRGGAAT'-3' and 803R 5'-CTACCRGGGTATCTAATCC-3') incorporating Roche 454 FLX Titanium adapters (Branford, CT) and a sample barcode sequence
Sponsors
NYU Langone Health
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Both men and women who have visited a gastroenterologist or surgeon, aged 18 or older will be included in this study. * Both men and women residents in the United States, aged 18 or older will be included in this study.
Exclusion criteria
* Women who are pregnant will be excluded from participating in this study * Subjects on ongoing antibiotic therapy will be excluded from participating in this study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Prevalence and relative abundance of selected fecal microbial taxa | 2 Days | Quantitative polymerase chain reaction |
Countries
United States
Outcome results
None listed