Dyslipidemias
Conditions
Keywords
fatty acids, biomarker, carbohydrate, lipogenesis
Brief summary
The overall aim is to investigate if circulating fatty acids and lipids are influenced by alterations in carbohydrate amount and quality.
Detailed description
In this isocaloric clinical cross-over trial, the investigators aim to investigate if circulating fatty acids and lipids are influenced by alterations in carbohydrate amount and quality. The investigators intend to evaluate if changes in carbohydrate quantity and quality (including sugar) influence circulating even-chain saturated fatty acids or if fatty acid-derived indices of desaturation and de novo lipogenesis are more sensitive biomarkers of carbohydrate intake. By performing untargeted lipidomic analyses, the investigators aim to search for novel lipid biomarkers of carbohydrate quantity and quality. The investigators also want to evaluate the influence of moderate alterations of carbohydrate and sugar intake on lipoproteins, insulin sensitivity, inflammatory proteins.
Interventions
OTHERLow carbohydrate diet
Diet low in carbohydrates (38%E), but higher in total and saturated fat.
OTHERHigh carbohydrate, high sugar diet
Diet high in carbohydrates (53%E) and sugars, but low in wholegrain, rye and wheat fibre.
OTHERHigh carbohydrate, low sugar diet
Diet high in carbohydrates (53%E), wholegrain, rye and wheat fibre, but low in sugars.
Sponsors
Uppsala University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI 20-38
Exclusion criteria
* abnormal lab tests (assessed by screening and judged by a physician), diabetes, cardiovascular, kidney, liver or malignant disease, and weight-loss (±3kg) the preceding 3 months of screening. Extreme diets, i.e. very-low carbohydrate diets, intermittent fasting, vegan diets or allergic to gluten
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Palmitic acid in different plasma fractions | 14-20 days | Measured by liquid gas chromatography (GC) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Even-chain saturated fatty acids (i.e. 14:0, 18:0) and monounsaturated fatty acids | 14-20 days | Measured by liquid GC |
Other
| Measure | Time frame | Description |
|---|---|---|
| Desaturation indices end lipogenic indices | 14-20 days | These indices are product-to-precursor fatty acids measured by GC |
Countries
Sweden
Outcome results
None listed