Healthy Volunteers
Conditions
Brief summary
The objectives of the study are 1) to characterize plasma and urinary metabolites that originate from glucobrassicin, 1-methoxyglucobrassicin, 4-methoxyglucobrassicin, and 4-hydroxyglucobrassicin, which are the major indole glucosinolates found in broccoli, and 2) to identify novel plasma and urinary metabolites following broccoli consumption that may have roles in reducing the risk of cancer.
Detailed description
Consumption of Brassica vegetables is inversely associated with incidence of several cancers, including cancer of the lung, stomach, liver, colon, rectum, breast, endometrium, and ovaries. Brassicas are a good source of many nutrients, but the unique characteristic of Brassicas is their rich content of glucosinolates. Breakdown products of indole glucosinolates in Brassica vegetables show promise for reducing the risk of cancer, but have received comparatively less research attention than those of aliphatic glucosinolates. The aim of this study is to investigate plasma and urinary metabolites of indole glucosinolates from broccoli and to identify novel metabolites that may have roles in reducing cancer risk.
Interventions
OTHERBroccoli consumption
All subjects will eat broccoli on day 11
Sponsors
USDA Beltsville Human Nutrition Research Center
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
21 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Non tobacco user * Cancer Free * Not currently taking glucosinolate/isothiocyanate containing supplements
Exclusion criteria
* Type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots * Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol, or Miradon (anisindione) * History of bariatric surgery or nutrient malabsorption disease (such as celiac disease) or other metabolic disorders requiring special diet recommendations * Pregnant, lactating, or intending to become pregnant during the study period * Crohn's disease or diverticulitis * Suspected or known strictures, fistulas or physiological/mechanical GI obstruction * Self-report of alcohol or substance abuse within the past 12 months and/or current acute treatment or rehabilitation program for these problems (long-term participation in Alcoholics Anonymous is not an exclusion)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The change in glucosinolate metabolites will be measured in blood plasma and urine. | On Day 11 | To track the changes of indole glucosinolate metabolites, glucosinolate metabolites will be measured in both blood plasma and urine. |
Countries
United States
Outcome results
None listed