Lung Cancer
Conditions
Brief summary
DNA adducts in the oral mucosa cells of 100 smokers from 3 ethnic groups - Native Hawaiians, Whites, and Japanese Americans with differing risks for lung cancer upon cigarette smoking will be quantified. DNA adducts of tobacco smoke carcinogens will be quantified using both targeted and untargeted approaches.
Detailed description
Using high resolution mass spectrometry, quantify known DNA adducts in oral mucosa cells of 100 smokers from each ethnic group - Native Hawaiians, Whites, and Japanese Americans. DNA adducts of tobacco-specific compounds, formaldehyde, and acrolein will be quantified. 2\. Analyze the urine of 100 smokers and 100 non-smokers from each of these groups for mercapturic acids of acrolein and crotonaldehyde, as well as total nicotine equivalents and total NNAL (smokers only). These data will provide critical information relevant to the high risk of Native Hawaiians for lung cancer, and in relationship to the DNA adduct measurements of Specific Aim 1.
Interventions
OTHERoral cell DNA adducts
oral cells will be collected, DNA isolated, and analyzed for adducts
Sponsors
University of Hawaii
University of Minnesota
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Inclusion criteria
* currently smoke at least 5 cigarettes per day, self-identified Japanese Americans, European Americans, or Native Hawaiians.
Exclusion criteria
* no current use of other nicotine containing products, no acute or uncontrolled medical or psychiatric conditions, no greater than 14 alcoholic drinks per week, not pregnant, not breastfeeding, not currently taking any medications that affect relevant metabolic enzymes, no active infection.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Concentration of γ-OH-Acr-dGuo, a Major Acrolein-DNA Adduct | Baseline | Acrolein-DNA adduct (8R/S)-3-(2'-deoxyribos-1'-yl)-5,6,7,8-tetrahy- dro-8-hydroxypyrimido\[1,2-a\]purine-10(3H)-one. Model adjusted for age, sex and race/ethnicity |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| εdAdo, Per 10^9 Nucleotides | Baseline | Lipid peroxidation-related adduct 1,N6-etheno-dAdo etheno-DNA adducts from peroxidation of lipids. Model adjusted for age, sex and race/ethnicity |
Countries
United States
Participant flow
Participants by arm
| Arm | Count |
|---|---|
| Native Hawaiian Cigarette Smokers oral cell DNA adducts
oral cell DNA adducts: oral cells will be collected, DNA isolated, and analyzed for adducts | 101 |
| White (European Americans) Cigarette Smokers oral cell DNA adducts
oral cell DNA adducts: oral cells will be collected, DNA isolated, and analyzed for adducts | 101 |
| Japanese American Cigarette Smokers oral cell DNA adducts
oral cell DNA adducts: oral cells will be collected, DNA isolated, and analyzed for adducts | 79 |
| Total | 281 |
Baseline characteristics
| Characteristic | Japanese American Cigarette Smokers | Total | Native Hawaiian Cigarette Smokers | White (European Americans) Cigarette Smokers |
|---|---|---|---|---|
| Age, Continuous | 59.2 years STANDARD_DEVIATION 14.6 | 50.1 years STANDARD_DEVIATION 16.1 | 45.5 years STANDARD_DEVIATION 17.7 | 47.5 years STANDARD_DEVIATION 15.7 |
| Race (NIH/OMB) American Indian or Alaska Native | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) Asian | 79 Participants | 79 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) Black or African American | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) More than one race | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) Native Hawaiian or Other Pacific Islander | 0 Participants | 101 Participants | 101 Participants | 0 Participants |
| Race (NIH/OMB) Unknown or Not Reported | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) White | 0 Participants | 101 Participants | 0 Participants | 101 Participants |
| Sex: Female, Male Female | 26 Participants | 128 Participants | 53 Participants | 49 Participants |
| Sex: Female, Male Male | 53 Participants | 153 Participants | 48 Participants | 52 Participants |
Adverse events
| Event type | EG000 affected / at risk | EG001 affected / at risk | EG002 affected / at risk |
|---|---|---|---|
| deaths Total, all-cause mortality | 0 / 0 | 0 / 0 | 0 / 0 |
| other Total, other adverse events | 0 / 0 | 0 / 0 | 0 / 0 |
| serious Total, serious adverse events | 0 / 0 | 0 / 0 | 0 / 0 |
Outcome results
Primary
Concentration of γ-OH-Acr-dGuo, a Major Acrolein-DNA Adduct
Acrolein-DNA adduct (8R/S)-3-(2'-deoxyribos-1'-yl)-5,6,7,8-tetrahy- dro-8-hydroxypyrimido\[1,2-a\]purine-10(3H)-one. Model adjusted for age, sex and race/ethnicity
Time frame: Baseline
| Arm | Measure | Value (MEAN) |
|---|---|---|
| Native Hawaiian Cigarette Smokers | Concentration of γ-OH-Acr-dGuo, a Major Acrolein-DNA Adduct | 49.7 10^9 nucleotides/mL |
| White (European Americans) Cigarette Smokers | Concentration of γ-OH-Acr-dGuo, a Major Acrolein-DNA Adduct | 67.1 10^9 nucleotides/mL |
| Japanese American Cigarette Smokers | Concentration of γ-OH-Acr-dGuo, a Major Acrolein-DNA Adduct | 46.5 10^9 nucleotides/mL |
Secondary
εdAdo, Per 10^9 Nucleotides
Lipid peroxidation-related adduct 1,N6-etheno-dAdo etheno-DNA adducts from peroxidation of lipids. Model adjusted for age, sex and race/ethnicity
Time frame: Baseline
| Arm | Measure | Value (MEAN) |
|---|---|---|
| Native Hawaiian Cigarette Smokers | εdAdo, Per 10^9 Nucleotides | 11.3 10^9 nucleotides/mL |
| White (European Americans) Cigarette Smokers | εdAdo, Per 10^9 Nucleotides | 11.3 10^9 nucleotides/mL |
| Japanese American Cigarette Smokers | εdAdo, Per 10^9 Nucleotides | 9.16 10^9 nucleotides/mL |