Effect of Platelet Storage on Complement Activation

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03280823

Enrollment

Registered

2017-09-13

Start date

2017-12-01

Completion date

2018-12-31

Last updated

2017-09-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Platelet Storage

Keywords

complement system

Brief summary

Platelet units are stored and complement factors are evaluated

Detailed description

Ten units of platelets will be selected from the regular inventory of the Central Blood Transfusion Services/Assiut University Hospitals . All units of platelets will be collected, processed, and tested following the standard procedures. Thesis units will be stored on a platelet rotator with the temperature controlled between 22 and 24c. Samples will be obtained using a sterile technique . The investigators will take basal sample and samples on day 2,3,4 and 5. Complement components will be assayed : C4 (classical pathway), C3 (common pathway) and C5 (terminal pathway). This evaluation will be done using ELISA technique

Interventions

None listed

Study design

Observational model

OTHER

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 45 Years

Inclusion criteria

* Negative screening and confirmatory tests will be done for all platelet donors

Exclusion criteria

* Use of aspirin or non steroidal anti-inflammatory drugs or medication affecting the platelets.

Design outcomes

Primary

Measure	Time frame
Platelets can be used after storage upto 5 days instead of using in the same day of donation without complement activation.	5 days

Contacts

Primary ContactShimaa A Ahmed

shimaaabdeleem11@gmail.com01065952778

Backup ContactMaha A Mahmoud

atwa-maha@yahoo.com01000004572

Outcome results