Cytokines, Inflammation Gum, Polycystic Ovary Syndrome
Conditions
Brief summary
Periodontal diseases are chronic inflammatory disease occurred by the interaction between pathogenic microorganism and the host defense. Polycystic ovary syndrome (PCOS) is a reproductive and metabolic disease associated with increased risk of cardiovascular events. Endocan is a proteoglycan secreted mainly by endothelial cells under the control of inflammatory cytokines. Periodontal diseases, including gingivitis, are common chronic infectious diseases caused by predominantly pathogenic microorganisms that colonize the subgingival area and cause local and systemic elevations of proinflammatory cytokines such as Interleukin-6 (IL-6). Several lines of evidence established the association between periodontal and systemic diseases, including metabolic syndrome, diabetes, and cardiovascular disease. Because of the fact that both periodontal disease and PCOS are associated with systemic inflammation and insulin resistance, these two disorders may be linked through a common pathophysiologic pathway. A number of studies have indicated a possible relationship between PCOS and periodontal inflammation. Despite common risk factors, including oxidative stress, the relationship between chronic periodontitis (CP) and PCOS remains unclear. The aims of the study were to determine serum and saliva Endocan and IL-6 levels and to evaluate the correlation between these two biomarker in women with periodontal disease and PCOS.
Interventions
DIAGNOSTIC_TESTCollection of Serum and Saliva Samples
Whole saliva samples were obtained via expectorating into polypropylene tubes venous blood were obtained from the antecubital vein via the standard venipuncture method.
Sponsors
Ataturk University
Ebru Saglam
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Newly diagnosed PCOS patients * Never smokers * Had no history of systemic disease * BMI\<25 kg/m2 * Participants had ≥20 teeth present.
Exclusion criteria
* Pregnancy * Lactation * Hemoglobin A1c (HbA1c) ≥ 6.5% * 2-h oral glucose tolerance test (OGTT-2h) ≥200 * Cushing syndrome, non-classic congenital adrenal hyperplasia, hyperprolactinemia, thyroid dysfunction, and androgen-secreting tumors * Using antibiotics and antiinflammatory within the past 6 months * Periodontal treatment within the past 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Serum and salivary Endocan levels | one year | Enzyme-Linked Immunosorbent Assay (ELISA) |
| Serum and salivary interleukin-6 levels | one year | Enzyme-Linked Immunosorbent Assay (ELISA) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Clinical attachment level (CAL) | one year | Periodontal measurement |
| Probing pocket depth (PD) | one year | Periodontal measurement |
| Gingival Index | One year | Periodontal measurement |
Countries
Turkey (Türkiye)
Outcome results
None listed